T7 RNA Polymerase

Name: T7 RNA Polymerase
Brand: Amid Biosciences
SKU: T7RP-301
Price: 70.0 USD
Availability: InStock

$ 70.00 USD

Sale Sold out

Quantity

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for its cognate promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript from a DNA sequence cloned downstream of the T7 promoter.

Applications:

Synthesis of mRNA for in vitro translation
Synthesis of guide RNA
Generation of antisense RNA
Generation of probes for nucleic acid hybridizations

Catalog # T7RP-301

For laboratory research use only.

Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene

Concentration: 50,000 U/ml

Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of CTP into acid-insoluble material in 1 hour at 37°C and pH 8.0 using linear DNA as the substrate

Storage buffer: 40 mM Tris-HCl (pH 7.9 at 25°C), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% (v/v) Triton® X-100 and 50% glycerol

Supplied reagents:

10X reaction buffer: (400 mM Tris-HCl, pH 7.9, 60 mM MgCl2, 100 mM Dithiothreitol, 20 mM Spermidine)

Storage: –20 °C

Item added to your cart

T7 RNA Polymerase

T7 RNA Polymerase

Country/region