Activity: 10,000 U/mL
Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
Supplied reagents:
- One vial (1mL) of 10X Reaction Buffer (200 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 20 mM DTT)
- One vial (0.2 mL) of 10 mM ATP
Unit assay conditions: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-phosphorylated 15-mer RNA into a phosphatase-resistant form in 30 minutes at 37°C in 1X T4 RNA Ligase 1 reaction buffer, supplemented with 1 mM ATP
Storage: T4 RNA ligase 1 and the supplied components must be stored at -20°C
