T4 RNA Ligase 1
T4 RNA Ligase 1
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T4 RNA Ligase 1 (ssRNA Ligase) catalyzes ATP-dependent ligation of single-stranded nucleic acids (RNA or DNA) by joining a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond.
Catalog # RNL1-301-1 (1000 Units)
This product is intended for laboratory research use only.
Activity: 10,000 U/mL
Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol
Supplied reagents:
- One vial (1mL) of 10X Reaction Buffer (200 mM Tris-HCl, pH 7.5, 100 mM MgCl2, 20 mM DTT)
- One vial (0.2 mL) of 10 mM ATP
Unit assay conditions: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-phosphorylated 15-mer RNA into a phosphatase-resistant form in 30 minutes at 37°C in 1X T4 RNA Ligase 1 reaction buffer, supplemented with 1 mM ATP
Storage: T4 RNA ligase 1 and the supplied components must be stored at -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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