Sortase A, Recombinant

Staphylococcus aureus Sortase A (srtA) belongs to a class of transpeptidases that utilize an active site cysteine thiol to modify proteins by recognizing and cleaving a carboxy-terminal sorting signal, LPXTG (where X is any amino acid), between the threonine and glycine residues. The terminal glycine is then replaced with any poly-Glycine (Gly)n label (where n= 3 or more glycine residues) to attach HPR, biotin, fluorophores and other labels. The enzyme is calcium dependent. 

Recombinant Staphylococcus aureus Sortase A is expressed in E. coli with an N-terminal His-tag.

Catalog # SRTA-301

This product is for research use only.

Target Protein (Donor): Genetically modified to have a C-terminal LPXTG motif.

Linker/Acceptor Molecule: Modified with a poly-glycine or aminomethylene tag