Recombinant Sortase A Enzyme for Protein Labeling | Amid Bio

Product Overview

Staphylococcus aureus Sortase A (srtA) is a recombinant transpeptidase engineered for highly efficient, site-specific protein engineering and bioconjugation. By recognizing and cleaving a carboxy-terminal sorting signal, LPXTG (where X is any amino acid) between the threonine and glycine residues, Sortase A forms a covalent acyl-enzyme intermediate.

The terminal glycine is subsequently replaced with a poly-Glycine (Gly)n nucleophile (where n ≥ 3), enabling precise, robust attachment of a wide range of labels—including HRP, biotin, fluorophores, and custom peptides.

This product is for research use only.

Key Features & Applications

• Site-Specific Labeling: Achieve homogenous modifications without disrupting the protein's native structure or activity.
• Versatile Bioconjugation: Ideal for antibody-drug conjugate (ADC) discovery, peptide ligation, and cell-surface labeling.
• Calcium-Dependent Activity: Standard catalytic activity relies on the presence of calcium ions (Ca2+).
• Easy Purification: Features an N-terminal 6×His-tag for straightforward downstream removal if required.

Technical Specifications

• Source: Staphylococcus aureus (Amino acids 26–206)
• Expression Host: E. coli
• Molecular Weight: 22.2 kDa
• Purity: > 90% by SDS-PAGE
• Storage Buffer: 20 mM HEPES (pH 7.5), 0.1 M NaCl, 20% glycerol