phi29 DNA Polymerase
phi29 DNA Polymerase
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phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). It is an extremely processive DNA polymerase (up to 70 kb per binding event) with extraordinary strand displacement capacity, exhibiting 3’ → 5’ proofreading exonuclease activity, resulting in an exceptionally high synthesis fidelity.
Catalog# PHDP-301
This product is for laboratory research use only.
Source: a recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Purity: >95% based on SDS-PAGE
Storage buffer: 10 mM Tris-HCl, pH 7.4 @ 25°C, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol
10X Phi29 DNA Polymerase Buffer: 500 mM Tris-HCl, pH 7.5 @ 25°C, 100 mM (NH4)2SO4, 40 mM dithiothreitol, 100 mM MgCl2
Unit definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C
International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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