Source: a recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29
Purity: >95% based on SDS-PAGE
Storage buffer: 10 mM Tris-HCl, pH 7.4 @ 25°C, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol
10X Phi29 DNA Polymerase Buffer: 500 mM Tris-HCl, pH 7.5 @ 25°C, 100 mM (NH4)2SO4, 40 mM dithiothreitol, 100 mM MgCl2
Unit definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C
