TG1 Competent Cells for High-efficiency phage display library construction and screening | Electrocompetent
High-efficiency Electrocompetent E. coli cells optimized for Phage display, Library construction, Library screening, Cloning, Subcloning, Protein expression with transformation efficiency ≥1 × 10^10 CFU/µg pUC19.
Available Sizes
What are TG1 Competent Cells?
TG1 competent cells are optimized E. coli host cells developed for high-efficiency High-efficiency phage display library construction and screening workflows including Phage display, Library construction, Library screening, Cloning, Subcloning, Protein expression. These cells support reliable plasmid uptake, stable propagation, and reproducible transformation performance for molecular biology and protein engineering applications.
Why Choose This Strain?
- Transformation efficiency: ≥1 × 10^10 CFU/µg pUC19
- Ultra-high efficiency
- Supports plasmids up to Phagemid-compatible; maximum plasmid size not specified in manual
- Stability: F′ episome with lacIq supports tight regulation of lac operator-controlled expression vectors
Key Features
- Strain: TG1
- Format: 10 × 0.05 mL/tube (20 reactions)
- Cell type: Electrocompetent
- Insert compatibility: Phage display and expression construct-dependent
- Optimized for High-efficiency phage display library construction and screening
Applications
- Phage display
- Library construction
- Library screening
- Cloning
- Subcloning
- Protein expression
Workflow Compatibility
- Electroporation of phage display libraries
- Antibody phage display workflows
- lac operator-regulated expression vectors
- T5/tac/trc promoter workflows
- High-efficiency TG1 transformation
Specifications
| Genotype | F’[traD36 lacIq lacZ ∆M15 proA+B+] glnV (supE) thi-1 ∆(mcrB-hsdSM)5 (rK- mK- McrB-) ∆(lac-proAB) |
|---|---|
| Antibiotic Resistance | Depends on transformed plasmid |
| Growth Conditions | SOC recovery; plate on selective LB agar with appropriate antibiotic |
| Plasmid Types | Phagemids, Phage display library plasmids, T5/tac/trc expression vectors, pUC19 |
| Recombination Notes | Not primarily a reduced-recombination cloning strain; optimized for phage display and regulated expression workflows |
| Max Plasmid Size | Phagemid-compatible; maximum plasmid size not specified in manual |
Detailed Specifications
| Stability Type | F′ episome with lacIq supports tight regulation of lac operator-controlled expression vectors |
|---|---|
| Insert Size Compatibility | Phage display and expression construct-dependent |
| Format | 10 × 0.05 mL/tube (20 reactions) |
Handling & Protocol
| Transformation Method | Electroporation |
|---|---|
| Protocol Time | ~1.5–2 hours to plating, plus 16–24 hour incubation |
| Recovery Time | 1 hour at 37°C in SOC |
| Storage Conditions | -80°C |
| Shipping Conditions | Dry ice |
| Shelf Life | ~6 - 12 months at -80°C (typical competent cell stability) |
Industry Comparison
Comparable to industry-standard TG1 electrocompetent phage display strains for High-efficiency phage display library construction and screening workflows.
Equivalent to industry-standard TG1 electrocompetent strains used for phage display library construction and screening, high-efficiency transformation, and lac-regulated expression workflows.
Any third-party trademarks are the property of their respective owners. Reference is provided solely to indicate compatibility or comparable application and does not imply affiliation, endorsement, or sponsorship.
Quality Control
- Transformation efficiency validated with control plasmid
- Genotype verified
- Contamination-free
- CoA provided
Key Advantages
- Ultra-high efficiency electrocompetent TG1 format
- Ideal for phage display library construction and screening
- Supports cloning, subcloning, and protein expression
- lacIq on F′ episome enables tight regulation of lac operator-controlled vectors
- Compatible with T5, tac, and trc promoter workflows
Why Researchers Choose Amid Biosciences Competent Cells
- High transformation efficiency for reliable cloning workflows
- Optimized strains for phage display, mutagenesis, and protein engineering
- Stringent quality control testing
- Fast shipping and scientific support
- Research-use-only products manufactured for reproducibility
Related Products
- SS320 Competent Cells for Large Phage Display Libraries
- TG1 Competent Cells for Antibody Phage Display
- DH5α Competent Cells for Routine Cloning
Research Use Only. Not for diagnostic or therapeutic use.