Aldehyde Activated Agarose Beads (4% Crosslinked)

High-Capacity Aldehyde Activated Agarose Beads

Amid Biosciences offers premium Aldehyde Agarose Beads (4% crosslinked), engineered for the stable, irreversible covalent immobilization of proteins, peptides, antibodies, and other biomolecules.

Unlike alternative coupling resins, these aldehyde-activated beads form an incredibly stable, uncharged secondary amine bond that prevents ligand leaching and minimizes non-specific binding during downstream affinity purification procedures.

Our aldehyde agarose beads arrive ready to use and require no pre-activation, significantly shortening your coupling workflow.

Key Specifications & Performance

• Matrix Support: 4% crosslinked agarose beads
• Coupling Efficiency: Typically >85% with standard proteins and antibodies
• Binding Capacity: 1 to 20 mg of immobilized protein per milliliter of resin
• Reaction Window: Fast 2 to 6 hour coupling time
• Buffer Compatibility: Simple, non-amine buffers (e.g., PBS or borate)
• Chemical Stability: Forms an uncharged, leak-resistant covalent bond superior to cyanogen bromide (CNBr) methods

Product Details & Advantages

• Target Primary Amines: Ideal for securely anchoring antibodies and enzymes via their primary amine (-NH2) groups.
• Flexible Coupling Conditions: Provides high efficiency across a wide pH range (4–10). Works seamlessly in standard PBS (pH 7.2) or enhanced citrate-carbonate (pH 10) environments, with or without organic solvents.
• Superior to CNBr-Activated Agarose: The resulting Schiff base bond—once stabilized with a mild reductant like sodium cyanoborohydride—is far more stable and leak-resistant than CNBr links, yielding cleaner purification results.
• Versatile & Reusable: Perfectly suited for both column (gravity-flow) and batch affinity techniques. The prepared matrix can be reused multiple times depending on the specific protein binding interactions.

Ordering Information

• Catalog # AA-101-5: 5 mL
• Catalog # AA-101-10: 10 mL
• Storage Conditions: Store at 2–8 °C. Do not freeze.

For research use only. Product requires shipping on ice packs.