Phage Display ER2738 Electrocompetent E. coli Cells

The E. coli strain ER2738 is an amber suppressor (glnV) F+ strain with a rapid growth rate and is particularly well-suited for M13 phage propagation. Amid Biosciences’ high efficiency electrocompetent ER2738 cells can be used for phage display, antibody phage display, library construction and screening. The F-factor of ER2738 contains a mini-transposon which confers tetracycline resistance, so cells can be selected by plating and propagating in tetracycline-containing medium. ER2738 cultures for infection can be grown either in LB or LB+Tet media. Tetracycline does not need to be added to media during phage amplification. Loss of F-factor in nonselective media is insignificant as long as cultures are not serially diluted. Although ER2738 is a recA+ strain, there are no reports on spontaneous in vivo recombination events with M13 or phagemid vectors.

ER2738 cells are recommended for use with New England Biolab′s Ph.D.™ Phage Display Kits (1).

ER2738 electrocompetent E. coli cells are supplied as a pack of 10 convenient 50 µl per tube aliquots (sufficient for 20 reactions).

Features and Benefits:

• Transformation efficiency > 10^10 transformants /µg pBR322
• Useful for preparation of phage display libraries
• Fast-growing
• Amber suppressor (supE): UAG>CAG(Gln)

Catalog # EREC-201

This product is intended for laboratory research use only.