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Klenow DNA Polymerase (3′ → 5′ exo-) for DNA Labeling

Klenow DNA Polymerase (3′ → 5′ exo-) for DNA Labeling

Regular price $ 90.00 USD
Regular price Sale price $ 90.00 USD
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Klenow DNA Polymerase (3′ → 5′ exo-) is the large fragment of E. coli DNA polymerase I. It exhibits 5'→3' polymerase activity, but lacks the 3'→5' (proofreading) and 5'→3' (nick-translation) exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site (D355A and E357A ). Polymerase incorporates modified nucleotides (e.g., fluorescein-, biotin-labeled nucleotides).

Applications:

  • Incorporation of fluorescent nucleotide analogs
  • A-tailing and T-tailing of adapters for Next Generation Sequencing
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • cDNA second-strand synthesis
  • Generate single-stranded DNA probes using random primers
  • Site-directed DNA mutagenesis using synthetic oligonucleotides

Catalog # KLE-601-250

This product is intended for laboratory research use only.