Klenow DNA Polymerase (3′ → 5′ exo-) for DNA Labeling

Klenow DNA Polymerase (3′ → 5′ exo-) is the large fragment of E. coli DNA polymerase I. It exhibits 5'→3' polymerase activity, but lacks the 3'→5' (proofreading) and 5'→3' (nick-translation) exonuclease activities of DNA Polymerase I. The 3'→5' exonuclease activity of the enzyme is eliminated by mutations in the 3'→5'-exonuclease active site (D355A and E357A ). Polymerase incorporates modified nucleotides (e.g., fluorescein-, biotin-labeled nucleotides).

Applications:

• Incorporation of fluorescent nucleotide analogs
• A-tailing and T-tailing of adapters for Next Generation Sequencing
• Dideoxy DNA sequencing of single- or double-stranded DNA templates
• cDNA second-strand synthesis
• Generate single-stranded DNA probes using random primers
• Site-directed DNA mutagenesis using synthetic oligonucleotides

Catalog # KLE-601-250

This product is intended for laboratory research use only.