Amid Biosciences | Protein Engineering Company
phi69 DNA Polymerase
phi29 DNA polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 (Φ29). It is an extremely processive DNA polymerase (up to 70 kb per binding event) with extraordinary strand displacement capacity, exhibiting 3’ → 5’ proofreading exonuclease activity, resulting in an exceptionally high synthesis fidelity.
Purity: >95% based on SDS-PAGE
Storage buffer: 10 mM Tris-HCl, pH 7.4 @ 25°C, 100 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.5% Tween-20, 0.5% NP-40, 50% glycerol.
10X Phi29 DNA Polymerase Buffer: 500 mM Tris-HCl, pH 7.5 @ 25°C, 100 mM (NH4)2SO4, 40 mM dithiothreitol, 100 mM MgCl2.
Unit Definition: 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTP's into acid insoluble material in 10 minutes at 30°C.
Source: A recombinant E. coli strain carrying the phi29 DNA Polymerase gene from bacteriophage phi29.
This product is for laboratory research use only.