GlycFree (λDE3) Chemically Competent E. coli Cells for Expression of Non-Glycosylated Recombinant Proteins

The GlycFree (λDE3) strain is the λDE3 lysogen of E. coli M226 strain (M226((λDE3)). It has been engineered for expression of sugar-free recombinant proteins in E. coli. Some glycosyltransferases have ability to catalyze a self-glycosylation reaction using UDP-glucose (UDPG) as glucose donor (1).  As such, these proteins can be aberrantly glycosylated during expression in common E.coli strains like BL21 and its derivatives. GlycFree (λDE3) is an E. coli strain defective in the synthesis of UDP-glucose. GlycFree(λDE3) allows to produce non-glycosylated forms of target proteins in native conformation and preserve their biological activity.

GlycFree (λDE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is suitable for controlled expression of both E. coli and T7 promoter driven constructs.

UDPG-mutant strains are not susceptible to phage P1 infection (2)

Catalog # GF-201

Highlights

• Expression of sugar-free recombinant proteins in E.coli.
• Transformation efficiency: >1 x 10⁵ cfu/µg pUC19
• Resistant to phage P1

E. coli genotype: e14- galU106 trp-50 relA1 spoT1 rpsL150 (str^R) (λDE3)

[λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Size: 10 X 0.1 ml/tube

Storage is recommended at -80°C.  

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Important Note: Purchase of GlycFree (λDE3) cells includes a license for use only at site of purchase and may not be distributed to other sites. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license at our Legal Communique Section before purchasing this product.

Product Manual

References

1. Chaikuad et al. Conformational plasticity of glycogenin and its maltosaccharide substrate during glycogen biogenesis. (2011) PNAS 108: 21028
2. Franklin N. Mutation in galU gene of E. coli blocks phage P1 infection. (1969) Virology 38(1): 189