Taq DNA Polymerase
Taq DNA Polymerase
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Taq DNA Polymerase is a thermostable DNA polymerase for everyday routine PCR. Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The enzyme lacks 3´ → 5´ exonuclease (proofreading) activity. Taq DNA polymerase’s terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product allows TA cloning of PCR products. Products up to 5 kb can be amplified from templates including genomic and plasmid DNA, bacterial colonies, and cDNA products. It is supplied with 10X Taq Reaction Buffer.
Features and benefits:
- Isolated from a recombinant source
- Non-proofreading DNA Polymerase
- Tolerates a wide range of templates
- Suitable for primer extension and TA cloning
- Robust and reliable performance at low price
Catalog # PC-TDP-601
This product is intended for laboratory use only.
10 X Reaction Buffer (with Mg^2+): 100 mM Tris HCl (pH 8.3 at 25°C), 500 mM KCl, 15 mM MgCl2
Concentration: Taq Polymerase is supplied as 5 units/µl in 20 mM Tris HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100 and 50% glycerol
Unit Definition: One unit is defined as the amount of enzyme that can incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C
Storage: -20°C. The enzyme can be shipped at room temperature without any loss of activity
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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