T4 RNA Ligase 2

Name: T4 RNA Ligase 2
Brand: Amid Biosciences
SKU: RNL2-301-1
Price: 90.0 USD
Availability: InStock

$ 90.00 USD

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T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity. T4 RNA Ligase 2 is more active for joining nicks on double stranded RNA than on joining the ends of single stranded RNA. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure. The non-ligated strand of the duplex can be either RNA or DNA. T4 RNA ligase 2 requires ATP for activity unless the substrate is preadenylated on the 5ʹ end.

Catalog #: RNL2-301-1 (200 Units), TVP-301-2 (1000 Units)

This product is intended for laboratory research use only.

Activity: 10,000 U/mL (>40,000 U/mg)

Storage buffer: 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% Glycerol

Supplied reagents:

One vial (1mL) of 10X Reaction Buffer (100 mM Tris-HCl, pH 7.5, 20 mM MgCl2, 20 mM DTT, 5 mM ATP)

Unit assay conditions: One unit is defined as the amount of enzyme required to ligate 50% of 0.4 µg of an equimolar mix of a single-stranded 15-mer RNA to the 5′ phosphorylated end of a 20-mer DNA in 20 µl 1X reaction buffer following a 30 minute incubation at 37°C

Storage: The enzyme and supplied components must be stored at -20°C

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T4 RNA Ligase 2

T4 RNA Ligase 2

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