T4 RNA Ligase 2

T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), exhibits high activity for both intermolecular and intramolecular RNA strand joining. Unlike standard T4 RNA ligases, T4 RNA Ligase 2 is significantly more efficient at joining nicks on double-stranded RNA (dsRNA) than at ligating the ends of single-stranded RNA (ssRNA).

Key Applications

• NGS Library Preparation: Ideal for RNA-seq and next-generation sequencing protocols requiring precise RNA strand joining.
• Double-Stranded RNA Nick Joining: Efficiently repairs nicks in dsRNA duplexes.
• RNA-to-DNA Ligation: Ligates the 3′-OH of RNA to the 5′-phosphate of DNA within a double-stranded hybrid structure (the non-ligated strand can be either RNA or DNA).

Technical Specifications

• Source: Recombinant protein expressed in E. coli.
• Concentration & Activity: 10,000 U/mL (>40,000 U/mg).
• Substrate Requirements: Requires an adjacent 5′ phosphate and 3′ OH for ligation. Requires ATP for activity unless the substrate is preadenylated on the 5′ end.
• Format & Catalog Numbers: Available in 200 Units (Catalog #: RNL2-301-1) and 1000 Units (Catalog #: RNL2-301-2).

Supplied Components & Storage

Each vial is supplied with 1 mL of 10X Reaction Buffer (100 mM Tris-HCl, pH 7.5, 20 mM MgCl₂, 20 mM DTT, 5 mM ATP).

Storage Conditions: Store at -20°C in its storage buffer (10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM DTT, 0.1 mM EDTA, 50% Glycerol).

This product is intended for laboratory research use only. For bulk orders, custom packaging, or international shipping logistics on ice packs, please contact info@amidbiosciences.com.