Protein Engineering Company
Cart 0

Amid Biosciences | Protein Engineering Company

Saposin B, Human, Recombinant | Amid Biosciences

$ 500.00

Saposin B is a small (79 amino acids), non-enzymatic glycosphingolipid activator protein required for the breakdown of cerebroside sulfates (sulfatides) in lysosomes. The protein can extract target lipids from membranes, forming soluble protein-lipid complexes that are recognized by arylsulfatase A.

Recombinant human saposin B is produced in E. coli as an N-terminal His-tag fusion and purified by chromatography techniques with subsequent removal of the tag trough a site-specific proteolytic cleavage. Saposins are glycosylated in a native state; however, non-glycosylated recombinant saposins produced in E. coli retain their respective activation effects in functional in vitro assays. 

Saposin B sequence

GDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDE

Catalog number: SAPB-301

Storage buffer: 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 50% Glycerol. 
Concentration: 1.0 mg/mL by A280 (E1% 3.4)
Purity: >90% by Coomassie staining 

Storage is recommended at -20°C for longer periods of time.  

This product is for laboratory research use only.

Product Information

 


Share this Product


More from this collection