Saposin C, Human, Recombinant

Saposin C (SAP-C) is a small, heat-stable lysosomal glycoprotein derived from the prosaposin precursor protein via site-specific proteolytic cleavage. Among the four prosaposin-derived domains, Saposin C shares high structural homology with Saposin A and acts as an indispensable biological cofactor required for the lysosomal activation of β-glucocerebrosidase (GBA)—the enzyme deficient in Gaucher disease. Additionally, Saposin C plays a critical role in cellular immunology by facilitating the presentation of lipid antigens through CD1b molecules to human T cells. While native lysosomal saposins are heavily glycosylated, non-glycosylated recombinant variants expressed in bacterial systems maintain full biological activation properties in functional in vitro biochemical assays.

Key Applications & SapC-DOPS Nanocarriers

Beyond its role in sphingolipid catabolism, recombinant Saposin C is widely utilized in translational oncology research. It can be assembled with dioleoylphosphatidylserine to form SapC-DOPS, a highly selective, membrane-targeted nanocarrier platform used to target and induce apoptosis in various cancer models, including glioblastoma multiforme. This makes high-purity, tag-free Saposin C an essential reagent for drug delivery innovation, lipid metabolism studies, and vaccine adjuvant formulation.

Product Features & Specifications

• Expression System: Engineered and expressed in an optimized Escherichia coli (E. coli) bacterial host.
• Tag Status: Expressed as an N-terminal His-tag fusion and purified using proprietary chromatography steps, followed by site-specific proteolytic cleavage to yield a completely tag-free final protein.
• High Purity: Verified at >90% purity by Coomassie blue staining on SDS-PAGE.
• Sequence Profile: Native mature Saposin C sequence (SDVYCEVCEF...SG).
• Formulation & Storage: Supplied at a concentration of 1.0 mg/mL (determined by A280) in 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 50% Glycerol. Store securely at -20°C.

Catalog Number: SAPC-302-1 | This product is intended for laboratory research use only (RUO). Product requires shipping on ice packs.