M-MLV Reverse Transcriptase for cDNA Synthesis
M-MLV Reverse Transcriptase for cDNA Synthesis
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Amid Biosciences' Reverse Transcriptase is an engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with a minimum RNase H activity and enhanced thermostability compared to wild-type M-MLV RT. The enzyme can copy a single-stranded DNA template or perform cDNA synthesis by initiating from a primer annealed to an RNA template. The optimal fist-strand cDNA synthesis temperature for this enzyme is 42°C, however, the enzyme is active up to 50o C, providing increased specificity, higher yields of cDNA, and more full-length product. It can generate cDNA up to 12 kb.
The enzyme is isolated from an E. coli strain overexpressing M-MLV RT gene construct.
Catalog # MRT-301
This product is intended for laboratory research use only
Size: 10,000 Units
Concentration: 200 u/μl
Storage Buffer: 20 mM Tris-HCl (pH 7.5), 1 mM DTT, 0.05% (v/v) Triton X-100, 0.1 mM EDTA, 0.1 M NaCl and 50% (v/v) glycerol
Reaction Buffer (5x): 250 mM Tris-HCl (pH 8.3 at room temperature), 375 mM KCl, 15 mM MgCl2
Unit Definition: One unit of the enzyme incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37˚C using poly (A):oligo (dT)25 as template-primer
Quality Control: This enzyme has passed the quality control assays: SDS-PAGE analysis for purity, functional absence of endonuclease activities, functional absence of exonuclease activities, functional absence of protease activity
Storage and Handling: -20° C
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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