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M-MLV Reverse Transcriptase for cDNA Synthesis

M-MLV Reverse Transcriptase for cDNA Synthesis

Regular price $ 120.00 USD
Regular price Sale price $ 120.00 USD
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Amid Biosciences' Reverse Transcriptase is an engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) with a minimum RNase H activity and enhanced thermostability compared to wild-type M-MLV RT. The enzyme can copy a single-stranded DNA template or perform cDNA synthesis by initiating from a primer annealed to an RNA template. The optimal fist-strand cDNA synthesis temperature for this enzyme is 42°C, however, the enzyme is active up to 50o C, providing increased specificity, higher yields of cDNA, and more full-length product. It can generate cDNA up to 12 kb.

The enzyme is isolated from an E. coli strain overexpressing M-MLV RT gene construct.

Catalog # MRT-301

This product is intended for laboratory research use only