High-Performance Reverse Transcriptase for cDNA Synthesis | Amid Biosciences
High-Performance Reverse Transcriptase for cDNA Synthesis | Amid Biosciences
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Amid Biosciences' Reverse Transcriptase is a high-efficiency, engineered version of the M-MLV enzyme, optimized for superior thermostability and significantly reduced RNase H activity. Designed for high-yield laboratory performance, this enzyme offers enhanced specificity and reliability for a variety of molecular biology applications.
Key Features & Benefits:
- Enhanced Heat Tolerance: While optimal at 42°C, this enzyme remains active up to 50°C, allowing for increased primer specificity and the ability to process complex RNA templates with high secondary structure.
- Long-Range Synthesis: Capable of generating full-length cDNA transcripts up to 12 kb, ensuring high-quality results even for long-form templates.
- Improved Yields: The reduction in RNase H activity prevents premature template degradation, resulting in higher yields of full-length products compared to standard wild-type enzymes.
- Versatile Template Usage: Efficiently synthesizes cDNA by initiating from primers annealed to RNA templates or by copying single-stranded DNA.
Catalog # MRT-301
For Research Use Only (RUO). This product is for research use only. It is not intended for diagnostic or therapeutic use in humans or animals.
Size: 10,000 Units
Concentration: 200 u/μl
Storage Buffer: 20 mM Tris-HCl (pH 7.5), 1 mM DTT, 0.05% (v/v) Triton X-100, 0.1 mM EDTA, 0.1 M NaCl and 50% (v/v) glycerol
Reaction Buffer (5x): 250 mM Tris-HCl (pH 8.3 at room temperature), 375 mM KCl, 15 mM MgCl2
Unit Definition: One unit of the enzyme incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37˚C using poly (A):oligo (dT)25 as template-primer
Quality Control: This enzyme has passed the quality control assays: SDS-PAGE analysis for purity, functional absence of endonuclease activities, functional absence of exonuclease activities, functional absence of protease activity
Storage and Handling: -20° C
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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