E. coli Poly(A) Polymerase | RNA Stabilization
E. coli Poly(A) Polymerase | RNA Stabilization
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E. coli Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3´-OH end of RNA molecules.
Applications:
- Addition of a poly(A) tail to RNA molecules to increase the stability of the RNA and enhance translation after transfection into eukaryotic cells
- Addition of a poly(A) tail to RNAs for cDNA synthesis
- Poly(A) tailing of RNA for cloning or affinity purification.
Catalog # PA-301
For laboratory research use only.
Source: Purified from a strain of E. coli that expresses the recombinant E.coli Poly (A) gene construct
Concentration: 5000 Units/ml
Unit definition: One unit of Poly(A) Polymerase catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C
Storage buffer: 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100, and 50% glycerol
Supplied reagents:
- 10X reaction buffer: 0.5 M Tris-HCl (pH 8.0), 2.5 M NaCl, and 100 mM MgCl2
Quality control: Poly(A) Polymerase is tested for polyadenylation of RNA in vitro. It is free of detectable RNase activity
Storage: –20°C
International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
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