E. coli Poly(A) Polymerase | RNA Stabilization

Source: Purified from a strain of E. coli that expresses the recombinant E.coli Poly (A) gene construct

Concentration: 5000 Units/ml

Unit definition: One unit of Poly(A) Polymerase catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C

Storage buffer: 50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100, and 50% glycerol

Supplied reagents:

• 10X reaction buffer: 0.5 M Tris-HCl (pH 8.0), 2.5 M NaCl, and 100 mM MgCl2

Quality control: Poly(A) Polymerase is tested for polyadenylation of RNA in vitro. It is free of detectable RNase activity

Storage: –20°C