TEV Protease, Recombinant, His-Tag | Affinity Tag Removal
TEV Protease, Recombinant, His-Tag | Affinity Tag Removal
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Amid Biosciences' TEV Protease is a 27 kDa engineered form of Tobacco Etch Virus (TEV) protease. The optimum recognition site for TEV protease is the seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] with cleavage occurring between the Gln and Gly/Ser residues. The enzyme is highly specific and active for its seven-amino acid sequence with minimal off-target effects which makes it an ideal choice to remove affinity tags from fusion proteins after protein purification. The optimal temperature for cleavage is 30°C; however, it can be used at temperature as low as 4°C. Recombinant TEV protease contains an N-terminal His tag and can be easily removed by immobilized metal affinity chromatography (IMAC) from the cleavage reaction. The enzyme is compatible for both in-solution and on-column cleavage reactions.
Features and Benefits:
- Highly specific cleavage activity
- Enhanced enzyme stability for prolonged protease activity
- Activity over a broad temperature (+4°C to 30°C) and pH (6.0 to 8.5) range
- His tag to facilitate its removal from the digested protein samples
Catalog # TVP-301-1 (1000 Units), TVP-301-5 (5000 Units)
This product is intended for laboratory research use only.
Source: E.Coli
Purity: >90% (SDS-PAGE)
Activity: 10,000 U/ml (~10,000 U/mg)
Unit definition: One unit of TEV Protease cleaves ≥85% of 3 µg control substrate in 1 hour at 30°C.
Supplied reagents:
- One vial of 20X TEV buffer (1 M Tris-HCl (pH 8.0), 10 mM EDTA)
- One vial of 100 mM DTT
Unit assay conditions: The cleavage assay is performed in the supplied components (buffer and DTT) with 1 unit of enzyme and 3 µg control substrate at 30°C for 1 hour in a total volume of 30 µl
Storage: Protease and the supplied components must be stored at -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates
References:
- Parks et al. Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology, 1995 Jun 20; 210(1): 194-201.
- Kapust et al. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng., 2001 Dec; 14(12): 993-1000.
- Cesaratto et al. Tobacco Etch Virus protease: A shortcut across biotechnologies. Journal of Biotechnology, 2016, 231, 239–249.
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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