pUC19 Plasmid Cloning Vector
pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). The pUC19 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene and contains unique sites for 13 different restriction enzymes (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I).
Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.
pUC19 plasmid DNA (10 μg or 100 μg) is provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
Copy Number: High (>100)
Origin of Replication: pMB1 (high-copy mutant)
Storage Temperature: -20°C
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This product is intended for laboratory research use only.
1. Vieira et al., The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 1982, 19 (30), 259 - 268