DNA Polymerase - Large Klenow Fragment
DNA Polymerase - Large Klenow Fragment
Couldn't load pickup availability
DNA Polymerase Large (Klenow) Fragment is a truncated fragment of E. coli DNA Polymerase I that lacks the 5´→ 3´ exonuclease activity but retains its 5´→ 3´ polymerase, 3´→ 5´ exonuclease (proofreading) and strand displacement activities.
Applications:
- Random primer labeling of DNA
- Labeling by fill-in 5'-overhangs of dsDNA
- Conversion of 5´-overhangs to create blunt-ends for cloning
- Removal of 3´-overhangs
- Second-strand synthesis of cDNA
- Site-directed DNA mutagenesis using synthetic oligonucleotides
- Dideoxy DNA sequencing of single- or double-stranded DNA templates
- Strand displacement amplification
Catalog # LKLE-601-250
This product is intended for laboratory research use only.
Enzyme concentration: 5 Units/ul
Unit Definition: One unit is defined as the amount of DNA Polymerase Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C under standard assay conditions.
Storage buffer: 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol
Optimal reaction temperature: 37 °C
Heat inactivation: 75 °C for 10 min
Enzyme supplied with 10X Reaction Buffer: 0.2 M Tris-HCl , pH 7.9, 0.5 M NaCl, 0.1 M MgCl2, 10 mM DTT
Storage: -20 °C
Shipping conditions: Shipped on dry ice
International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
- Choosing a selection results in a full page refresh.
- Opens in a new window.