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DNA Polymerase - Large Klenow Fragment

DNA Polymerase - Large Klenow Fragment

Regular price $ 90.00 USD
Regular price Sale price $ 90.00 USD
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DNA Polymerase Large (Klenow) Fragment is a truncated fragment of E. coli DNA Polymerase I that lacks the 5´→ 3´ exonuclease activity but retains its 5´→ 3´ polymerase, 3´→ 5´ exonuclease (proofreading) and strand displacement activities.

Applications:

  • Random primer labeling of DNA
  • Labeling by fill-in 5'-overhangs of dsDNA
  • Conversion of 5´-overhangs to create blunt-ends for cloning
  • Removal of 3´-overhangs
  • Second-strand synthesis of cDNA
  • Site-directed DNA mutagenesis using synthetic oligonucleotides
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • Strand displacement amplification

Catalog # LKLE-601-250

This product is intended for laboratory research use only.