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Large Klenow DNA Polymerase | DNA end filling | Genomic Libraries Preparation

Amid Biosciences| Competent Cells and Protein Expression Vectors

DNA Polymerase - Large Klenow Fragment

$ 90.00

DNA Polymerase Large (Klenow) Fragment is a truncated fragment of E. coli DNA Polymerase I that lacks the 5´→ 3´ exonuclease activity but retains its 5´→ 3´ polymerase, 3´→ 5´ exonuclease (proofreading) and strand displacement activities. 



  • Random primer labeling of DNA
  • Labeling by fill-in 5'-overhangs of dsDNA
  • Conversion of 5´-overhangs to create blunt-ends for cloning
  • Removal of 3´-overhangs
  • Second-strand synthesis of cDNA
  • Site-directed DNA mutagenesis using synthetic oligonucleotides
  • Dideoxy DNA sequencing of single- or double-stranded DNA templates
  • Strand displacement amplification


Enzyme concentration: 5 Units/ul

Storage buffer: 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol

Optimal reaction temperature: 37 oC

Heat inactivation: 75 °C for 10 min

Storage: -20 oC

Shipping conditions: Shipped on dry ice

International Shipping:  Product requires shipping on dry ice. Please contact for shipment estimates. 

Enzyme supplied with 10X Reaction Buffer (0.2 M Tris-HCl , pH 7.9, 0.5 M NaCl, 0.1 M MgCl2 , 10 mM DTT)

Unit Definition: One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C under standard assay conditions.


This product is intended for laboratory research use only. Not for diagnostic purposes.

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