KRAS Wild Type Protein, Human, Recombinant, Biotinylated, GDP-Loaded

N-terminal AviTag™, N-terminal 8XHis

High-purity KRAS Wild Type protein supplied in a defined GDP-loaded conformation for controlled biochemical workflows, including SPR, BLI, ELISA, pull-down, AlphaScreen, MST, nucleotide exchange assays, SOS1-mediated exchange assays, and oncology drug discovery applications.

Available Sizes

0.05 mg
$ 500.00
0.1 mg
$ 750.00
1.0 mg
$ 5,500.00

Ships with Certificate of Analysis (CoA)

Product Overview

Recombinant human KRAS WT (isoform KRAS4B, aa 1–185), site-specifically biotinylated at the N-terminal AviTag™ and pre-loaded with GDP. The GDP-loaded inactive conformation is the physiologically relevant resting state of KRAS WT, in which GTP hydrolysis is efficient (unlike oncogenic mutants) and SOS1-catalyzed exchange is the rate-limiting activation step.

Biotinylated KRAS WT in the GDP state is particularly useful for nucleotide exchange assays: SOS1 activity can be monitored by fluorescent nucleotide exchange (e.g., BODIPY-FL-GDP or mantGDP displacement) after capturing the protein on a streptavidin surface or in solution. The site-specific biotin avoids modification of exchange-relevant residues.

Key Features

  • KRAS endogenous signaling control protein
  • Supplied pre-loaded with GDP
  • N-terminal AviTag™, N-terminal 8XHis tagged construct
  • Site-specifically biotinylated at the N-terminus
  • High purity recombinant protein
  • Optimized for binding assays
  • Research Use Only (RUO)

Applications

  • SPR
  • BLI
  • ELISA
  • Pull-down
  • AlphaScreen
  • MST
  • Nucleotide exchange assays
  • SOS1-mediated exchange assays
  • Protein–protein interaction studies
  • Drug discovery workflows

Technical Notes

WT KRAS hydrolyzes GTP efficiently — do not use with live GTP

Unlike oncogenic mutants, WT KRAS has intact GTPase activity (T35 and G60 positions are WT). If you add live GTP to this protein, it will hydrolyze back to GDP within minutes. For applications requiring the active conformation of WT KRAS, use the GppNHp-loaded version which is hydrolysis-resistant.

SOS1 exchange assay compatibility

GDP-loaded KRAS WT is the preferred substrate for SOS1 GEF assays. SOS1 catalyzes release of bound GDP and loading of GTP (or fluorescent GTP analogues). For a standard fluorescent exchange assay, pre-incubate KRAS WT GDP-loaded with BODIPY-FL-GDP at low concentration to label a fraction of molecules, then add SOS1 and monitor fluorescence change as labeled GDP is displaced by excess unlabeled GTP.

Biological Context & Applications

Protein Specifications

Gene KRAS
Mutation Wild-type
Nucleotide State GDP-Loaded
Organism Human
Tag N-terminal AviTag™, N-terminal 8XHis
Expression System E. coli
Purity >90% by Coomassie staining
Endotoxin <1 EU/mg
Biotinylated Yes
Oncogenic Status Wild Type
Assay Compatibility SPR, BLI, ELISA, Pull-down, AlphaScreen, MST, Nucleotide exchange assays, SOS1-mediated exchange assays
UniProt ID P01116-2
Amino Acid Range 1-185
Buffer 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol
Storage -80 °C

Quality Control

  • Purity validated by SDS-PAGE
  • Identity confirmed by mass spectrometry
  • Assay compatibility verified
  • A Certificate of Analysis (CoA) is provided with every shipment

Ordering Information

Size Catalog No. Price Quote
0.05 mg KRAS-301-GDP-005 $ 500.00 Request Quote
0.1 mg KRAS-301-GDP-01 $ 750.00 Request Quote
1.0 mg KRAS-301-GDP-1 $ 5,500.00 Request Quote
Need larger quantities or custom formats? We offer bulk pricing and custom production.
Request Bulk Quote

Typical response time: 1 business day

Custom Services

Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.

Custom Mutations

Alternative KRAS variants, including rare and novel mutations.

Tag & Labeling Options

His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.

Scale-Up Production

From research-scale to bulk manufacturing for screening and preclinical work.

Protein Complexes

Complex formation such as KRAS–RAF-RBD and other binding partners.

Nucleotide Loading

Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.

Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.

Request Custom Quote Email Us

Fast turnaround • Dedicated scientific support • Confidential projects

Related Products

Other KRAS mutants and formats available.

Research Use Only. Not for diagnostic or therapeutic use.