KRAS G12V Protein, Human, Recombinant, Biotinylated, GDP-Loaded

N-terminal AviTag™, N-terminal 8XHis

High-purity KRAS G12V protein supplied in a defined GDP-loaded conformation for controlled biochemical workflows, including SPR, BLI, ELISA, pull-down, AlphaScreen, MST, nucleotide exchange assays, SOS1-mediated exchange assays, and oncology drug discovery applications.

Available Sizes

0.05 mg
$ 500.00
0.1 mg
$ 750.00
1.0 mg
$ 5,500.00

Ships with Certificate of Analysis (CoA)

Product Overview

Recombinant human KRAS G12V (isoform KRAS4B, aa 1–185), site-specifically biotinylated at the N-terminal AviTag™ and pre-loaded with GDP. The GDP-loaded conformation enables studies of the inactive-state G12V protein, including pocket geometry characterization, inhibitor binding to any GDP-state pockets, and use as a selectivity control in G12C and G12D inhibitor panels.

KRAS G12V GDP-loaded also serves as a substrate for SOS1-catalyzed nucleotide exchange, although the exchange rate may differ from WT due to altered switch region dynamics.

Key Features

  • KRAS G12V clinically relevant mutation
  • Supplied pre-loaded with GDP
  • N-terminal AviTag™, N-terminal 8XHis tagged construct
  • Site-specifically biotinylated at the N-terminus
  • High purity recombinant protein
  • Optimized for binding assays
  • Research Use Only (RUO)

Applications

  • SPR
  • BLI
  • ELISA
  • Pull-down
  • AlphaScreen
  • MST
  • Nucleotide exchange assays
  • SOS1-mediated exchange assays
  • Protein–protein interaction studies
  • Drug discovery workflows

Technical Notes

G12V impairs GTP hydrolysis more severely than G12D

The valine at position 12 creates steric clash with the catalytic glutamine (Q61) and the arginine finger of GAP proteins, impairing intrinsic GTPase activity more severely than G12D. In cells, KRAS G12V is more constitutively GTP-loaded than G12D. In vitro, both proteins are supplied in the GDP state for practical reasons (stability), and nucleotide exchange is required to access the active state.

Pocket geometry differs from G12C and G12D

The G12V mutation creates a different switch II pocket geometry compared to G12C and G12D, which is why G12C-selective covalent inhibitors do not bind G12V and G12D-selective compounds (e.g., MRTX-1133) have reduced potency against G12V. Do not assume inhibitor cross-reactivity between mutants without experimental confirmation.

Biological Context & Applications

Cancer Indications

Colorectal cancer, pancreatic cancer, non-small cell lung cancer, lung adenocarcinoma, gastric cancer, esophageal cancer

Binding Partners

RAF1, RalGDS, PI3K, BRAF

Application Notes

Strong constitutive activation; ideal for functional and effector-binding assays

Protein Specifications

Gene KRAS
Mutation G12V
Nucleotide State GDP-Loaded
Organism Human
Tag N-terminal AviTag™, N-terminal 8XHis
Expression System E. coli
Purity >90% by Coomassie staining
Endotoxin <1 EU/mg
Biotinylated Yes
Oncogenic Status Oncogenic
Assay Compatibility SPR, BLI, ELISA, Pull-down, AlphaScreen, MST, Nucleotide exchange assays, SOS1-mediated exchange assays
UniProt ID P01116-2
Amino Acid Range 1-185
Buffer 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol
Storage -80 °C

Quality Control

  • Purity validated by SDS-PAGE
  • Identity confirmed by mass spectrometry
  • Assay compatibility verified
  • A Certificate of Analysis (CoA) is provided with every shipment

Ordering Information

Size Catalog No. Price Quote
0.05 mg KRAS12V-GDP-301-005 $ 500.00 Request Quote
0.1 mg KRAS12V-GDP-301-01 $ 750.00 Request Quote
1.0 mg KRAS12V-GDP-301-1 $ 5,500.00 Request Quote
Need larger quantities or custom formats? We offer bulk pricing and custom production.
Request Bulk Quote

Typical response time: 1 business day

Custom Services

Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.

Custom Mutations

Alternative KRAS variants, including rare and novel mutations.

Tag & Labeling Options

His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.

Scale-Up Production

From research-scale to bulk manufacturing for screening and preclinical work.

Protein Complexes

Complex formation such as KRAS–RAF-RBD and other binding partners.

Nucleotide Loading

Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.

Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.

Request Custom Quote Email Us

Fast turnaround • Dedicated scientific support • Confidential projects

Related Products

Other KRAS mutants and formats available.

Research Use Only. Not for diagnostic or therapeutic use.