KRAS G12D Protein, Human, Recombinant, Biotinylated, GDP-Loaded

N-terminal AviTag™, N-terminal 8XHis

High-purity KRAS G12D protein supplied in a defined GDP-loaded conformation for controlled biochemical workflows, including SPR, BLI, ELISA, pull-down, AlphaScreen, MST, nucleotide exchange assays, SOS1-mediated exchange assays, and oncology drug discovery applications.

Available Sizes

0.05 mg
$ 500.00
0.1 mg
$ 750.00
1.0 mg
$ 5,500.00

Ships with Certificate of Analysis (CoA)

Product Overview

This product provides recombinant human KRAS G12D protein site-specifically biotinylated at the N-terminal AviTag™ and pre-loaded with GDP to support studies of the GDP-bound inactive-state conformation. The combination of site-specific biotinylation and defined nucleotide state removes two common preparation steps from your workflow: streptavidin capture assays can be set up directly, and the protein is ready for SOS1-mediated nucleotide exchange assays without pre-loading. Researchers may exchange the protein to GTP or non-hydrolyzable GTP analogs such as GppNHp, GMPPNP, or GTPγS depending on assay requirements.

Key Features

  • KRAS G12D clinically relevant mutation
  • Supplied pre-loaded with GDP
  • N-terminal AviTag™, N-terminal 8XHis tagged construct
  • Site-specifically biotinylated at the N-terminus
  • High purity recombinant protein
  • Optimized for binding assays
  • Research Use Only (RUO)

Applications

  • SPR
  • BLI
  • ELISA
  • Pull-down
  • AlphaScreen
  • MST
  • Nucleotide exchange assays
  • SOS1-mediated exchange assays
  • Protein–protein interaction studies
  • Drug discovery workflows

Technical Notes

On nucleotide loading and the GDP conformation

GDP-loaded KRAS G12D represents the biologically inactive state. While the G12D mutation impairs GTP hydrolysis and favors GTP-bound (active) KRAS in cells, the recombinant protein is supplied GDP-loaded for stability and experimental flexibility. Researchers can initiate exchange to GTP (or non-hydrolyzable analogues such as GppNHp, GMPPNP, or GTPγS) using SOS1 as a guanine nucleotide exchange factor. For assays requiring constitutively active KRAS G12D, please see our GppNHp-loaded version.

On N-terminal biotinylation and effector binding

AviTag biotinylation is at the N-terminus, upstream of the KRAS catalytic domain (aa 10–164). The switch I (aa 30–38) and switch II (aa 60–76) regions that mediate effector binding are in the core domain and are not directly affected by N-terminal tag placement. However, users performing steric-sensitive assays (e.g., antibody epitope mapping near the N-terminus) may prefer C-terminally biotinylated constructs — see KRAS G12V (1–166), Biotinylated at C-terminus (Cat #KRAS12V-B-C-301) for a reference format; C-terminal G12D is available on request.

MgCl₂ is required for nucleotide retention

The storage buffer contains 1 mM MgCl₂, which is essential for Mg²⁺-GDP coordination at the nucleotide binding site. Assay buffers should maintain 1–5 mM MgCl₂. Removal of Mg²⁺ leads to rapid nucleotide dissociation. EDTA should not be used in working buffers.

Biological Context & Applications

Cancer Indications

Pancreatic ductal adenocarcinoma (PDAC), colorectal cancer, non-small cell lung cancer, appendiceal cancer, cholangiocarcinoma, endometrial cancer

Binding Partners

RAF1, PI3K, SOS1, RalGDS

Application Notes

Core mutation for oncology drug discovery workflows

Protein Specifications

Gene KRAS
Mutation G12D
Nucleotide State GDP-Loaded
Organism Human
Tag N-terminal AviTag™, N-terminal 8XHis
Expression System E. coli
Purity >90% by Coomassie staining
Endotoxin <1 EU/mg
Biotinylated Yes
Oncogenic Status Oncogenic
Assay Compatibility SPR, BLI, ELISA, Pull-down, AlphaScreen, MST, Nucleotide exchange assays, SOS1-mediated exchange assays
UniProt ID P01116-2
Amino Acid Range 1-185
Buffer 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol
Storage -80 °C

Quality Control

  • Purity validated by SDS-PAGE
  • Identity confirmed by mass spectrometry
  • Assay compatibility verified
  • A Certificate of Analysis (CoA) is provided with every shipment

Ordering Information

Size Catalog No. Price Quote
0.05 mg KRAS12D-GDP-301-005 $ 500.00 Request Quote
0.1 mg KRAS12D-GDP-301-01 $ 750.00 Request Quote
1.0 mg KRAS12D-GDP-301-1 $ 5,500.00 Request Quote
Need larger quantities or custom formats? We offer bulk pricing and custom production.
Request Bulk Quote

Typical response time: 1 business day

Custom Services

Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.

Custom Mutations

Alternative KRAS variants, including rare and novel mutations.

Tag & Labeling Options

His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.

Scale-Up Production

From research-scale to bulk manufacturing for screening and preclinical work.

Protein Complexes

Complex formation such as KRAS–RAF-RBD and other binding partners.

Nucleotide Loading

Pre-load KRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.

Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.

Request Custom Quote Email Us

Fast turnaround • Dedicated scientific support • Confidential projects

Related Products

Other KRAS mutants and formats available.

Research Use Only. Not for diagnostic or therapeutic use.