HRV 3C Protease, Recombinant, HisTag | Affinity Tag Removal
HRV 3C Protease, Recombinant, HisTag | Affinity Tag Removal
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Amid Biosciences HRV 3C protease is a recombinant cysteine protease derived from human Rhinovirus type 14. The enzyme is known as PreScission Protease. HRV 3C protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro. Besides the recognition sequence, the secondary and tertiary structures of the fusion protein influence on substrate recognition and cleavage.
The recombinant enzyme is a 21 kDa protein with polyhistidine (6xHis) tag, so it can be removed from the protease reaction solution through immobilized metal affinity chromatography (IMAC).
Benefits:
- Low temperature cleavage of fusion proteins.
- Active in a variety of commonly used buffers.
- On-column or off-column cleavage
Applications:
- Removal of purification tags from fusion proteins.
Catalog # HRV3C-301-1
This product is intended for laboratory research use only.
Activity: ≥1000U/mg
Concentration: 1.1 mg/mL
Unit Definition: One unit of HRV 3C Protease is defined as the amount of enzyme that cleaves ≥95% of the control fusion protein (0.1 mg in 20 mM Tris-HCl, 100 mM NaCl, pH 7.5, and 2 mM 2-mercaptoethanol) in 16 hours at 4°C
Physical form: Supplied in a solution containing 20 mM Tris HCl (pH 7.5), 0.1 M NaCl, 2 mM 2-mercaptoethanol, 0.04% Tween 20 and 50% (V/V) glycerol
Storage: at -20°C
International Shipping: Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates
Domestic Shipping in the US
Temperature sensitive products requiring shipping/handling on dry ice are shipped via UPS Next Day Air Saver. These products are delivered the next day if your order is placed before 3 PM PST. A dry ice surcharge is included in the shipping cost at checkout. For orders totaling over $1,000, free shipping and handling will be applied to the order.
International Shipping
Orders to be shipped internationally (outside of the US) can be shipped via DHL Express Worldwide or FedEx International Priority. Please contact info@amidbiosciences.com if you would like your company's shipping account to be used for the order. Our customers have sometimes negotiated lower shipping rates than us.
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