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TEV Protease, Recombinant, His-Tag for Affinity Tag Removal

Amid Biosciences| Competent Cells and Protein Expression Vectors

TEV Protease, Recombinant, His-Tag | Affinity Tag Removal

$ 90.00

Amid Biosciences TEV Protease is a 27 kDa engineered form of Tobacco Etch Virus (TEV) protease. The optimum recognition site for TEV protease is the seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] with cleavage occurring between the Gln and Gly/Ser residues. The enzyme is highly specific and active for its seven-amino acid sequence with minimal off-target effects which makes it an ideal choice to remove affinity tags from fusion proteins after protein purification. The optimal temperature for cleavage is 30°C; however, it can be used at temperature as low as 4°C. Recombinant TEV protease contains an N-terminal His tag and can be easily removed by immobilized metal affinity chromatography (IMAC) from the cleavage reaction. The enzyme is compatible for both in-solution and on-column cleavage reactions. TEV Protease is purified from E. coli by proprietary chromatographic techniques and is >90% pure when visualized on an SDS-PAGE gel.

TEV Protease is supplied with a vial of 20X TEV buffer (1 M Tris-HCl (pH 8.0), 10 mM EDTA) and a vial of 100 mM DTT.

  • Highly specific cleavage activity
  • Enhanced enzyme stability for prolonged protease activity
  • Activity over a broad temperature (+4°C to 30°C) and pH (6.0 to 8.5) range
  • His tag to facilitate its removal from the digested protein samples

Activity: 10,000 U/ml (~10,000 U/mg)

SKU # TVP-301-1 (1000 Units), TVP-301-5 (5000 Units).

Unit Definition

One unit of TEV Protease cleaves ≥85% of 3 µg control substrate in 1 hour at 30°C.

Unit Assay Conditions

The cleavage assay is performed in 1X TEV Buffer (50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA) and 1 mM DTT with 1 unit of enzyme  and 3 µg control substrate at 30°C for 1 hour in a total volume of 30 µl.

Storage: Protease and the supplied components must be stored at -20°C.

International Shipping:  Product requires shipping on ice packs. Please contact for shipment estimates

This product is intended for laboratory research use only.

TEV Protease Product Manual


 1. Parks et al. Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form. Virology, 1995 Jun 20; 210(1): 194-201.

2. Kapust et al. Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency. Protein Eng., 2001 Dec; 14(12): 993-1000.

3. Cesaratto et al. Tobacco Etch Virus protease: A shortcut across biotechnologies. Journal of Biotechnology, 2016, 231, 239–249.


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