Recombinant Human HRAS Wild Type Protein (N-terminal AviTag™, N-terminal 6XHis )
High-purity HRAS Wild Type for SPR, BLI, ELISA, Pull-down, AlphaScreen, MST and oncology drug discovery.
Available Sizes
Product Overview
The recombinant HRAS Wild Type protein is designed for high-confidence binding and interaction studies. This construct supports site-specific biotinylation for surface-based assays.
Key Features
- HRAS endogenous signaling control protein
- N-terminal AviTag™, N-terminal 6XHis tagged construct
- Supports site-specific biotinylation
- High purity recombinant protein
- Optimized for binding assays
- Research Use Only (RUO)
Applications
- SPR
- BLI
- ELISA
- Pull-down
- AlphaScreen
- MST
- Protein–protein interaction studies
- Drug discovery workflows
Biological Context & Applications
Cancer Indications
Head and neck squamous cell carcinoma (HNSCC), bladder cancer, thyroid cancer, skin cancers
Binding Partners
RAF1, PI3K, SOS1, RalGDS
Application Notes
Useful for comparative RAS isoform studies and signaling pathway analysis
Protein Specifications
| Gene | HRAS |
|---|---|
| Mutation | Wild-type |
| Organism | Human |
| Tag | N-terminal AviTag™, N-terminal 6XHis |
| Expression System | E. coli |
| Purity | >90% by Coomassie staining |
| Endotoxin | <1 EU/mg |
| Biotinylated | Yes |
| Oncogenic Status | Wild Type |
| Assay Compatibility | SPR, BLI, ELISA, Pull-down, AlphaScreen, MST |
| UniProt ID | P01112 |
| Amino Acid Range | 1-189 |
| Buffer | 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 1 mM 2-mercapthoethanol, 10% glycerol |
| Storage | -80 °C |
Quality Control
- Purity validated by SDS-PAGE
- Identity confirmed by mass spectrometry
- Assay compatibility verified
- A Certificate of Analysis (CoA) is provided with every shipment
Ordering Information
| Size | Catalog No. | Price | Quote |
|---|---|---|---|
| 0.1 mg | HRAS-B-301 | $ 800.00 | Request Quote |
Typical response time: 1 business day
Custom Services
Need a different format, mutation, or production scale? We offer custom RAS protein engineering tailored to your research.
Alternative HRAS variants, including rare and novel mutations.
His-tag, AviTag™, biotinylation, fluorescent labeling, or tag-free formats.
From research-scale to bulk manufacturing for screening and preclinical work.
Complex formation such as HRAS–RAF-RBD and other binding partners.
Pre-load HRAS proteins with defined nucleotides, including GppNHp (non-hydrolyzable GTP analog) or GDP, for consistent assay-ready performance.
Tell us your mutation, format, or assay requirements and we’ll provide a tailored quote.
Fast turnaround • Dedicated scientific support • Confidential projects
Related Products
Other HRAS mutants and formats available.
Research Use Only. Not for diagnostic or therapeutic use.