Rhamnose-Inducible Expression Vector - pTrham

Amid Biosciences’ pTrham vector produces the highest yields of recombinant proteins compared to other rhamnose expression vectors available on the market. This plasmid-based expression system features optimized transcription and translation regulatory elements, delivering high-level production of heterologous proteins in a correct, bioactive conformation upon induction with L-rhamnose, while maintaining extremely low basal expression levels in its absence.

Key Features & Benefits

• Tightly Regulated Expression: Controlled at the individual cell level, making it ideal for the stable cloning and high-yield expression of toxic proteins.
• Enhanced Translation Efficiency: The Escherichia coli L-rhamnose-inducible rhaBAD promoter is complemented by the rrnG anti-termination region, the bacteriophage T7 gene 10 translation enhancer, and a robust ribosome binding site (RBS).
• Broad Compatibility: The pTrham expression vector functions efficiently across any E. coli strain.
• Cloning-Friendly Size: At 3.3 kb, it facilitates easy cloning of large inserts and routine DNA manipulations like site-directed mutagenesis.
• Versatile Directional Cloning: A comprehensive Multiple Cloning Site (MCS) provides flexible options using a variety of standard restriction enzymes.

Product Specifications

• Catalog #: PTR-401
• Size: 5 µg
• Storage Conditions: Store at -20°C
• Application: For Research Use Only (RUO). Not intended for diagnostic or therapeutic use in humans or animals.

Product Citations in Peer-Reviewed Literature

• Weyant, K.B., Oloyede, A., Pal, S. et al. A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens. Nat Commun 14, 464 (2023). https://doi.org/10.1038/s41467-023-36101-2
• Weyant, K.B., Oloyede, A., & DeLisa, M.P. On-Demand Vaccine Production via Dock-and-Display of Biotinylated Antigens on Bacterial Extracellular Vesicles. Methods in Molecular Biology, Springer, 195–216 (2024). https://doi.org/10.1007/978-1-0716-4055-5_13

Please Note: Purchase of the pTrham expression vector includes a limited-use license for the site of purchase only. Distribution, assignment, or transfer to any third party is prohibited without express written consent from Amid Biosciences, LLC.