Amid Biosciences | Protein Engineering Company
T7 RNA Polymerase
T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for its cognate promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript from a DNA sequence cloned downstream of the T7 promoter.
Applications
- Synthesis of mRNA for in vitro translation.
- Synthesis of guide RNA.
- Generation of antisense RNA.
- Generation of probes for nucleic acid hybridizations.
Storage buffer: 40 mM Tris-HCl (pH 7.9 at 25°C), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% (v/v) Triton® X-100 and 50% glycerol.
The enzyme is supplied with 10X T7 RNA Polymerase Buffer (400 mM Tris-HCl, pH 7.9, 60 mM MgCl2, 100 mM Dithiothreitol, 20 mM Spermidine).
Catalog number: T7RP-301
Concentration: 50,000 U/ml
Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of CTP into acid-insoluble material in 1 hour at 37°C and pH 8.0 using linear DNA as the substrate.
Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.
Storage: –20 °C
Usage: For laboratory research use only.
