VAV1 Protein, Human, Recombinant, Biotinylated

The guanine nucleotide exchange factor (GEF) VAV1 plays an important role in T-cell activation and tumorigenesis. In the GEF superfamily, VAV1 has the ability to interact with multiple families of Rho GTPases. VAV1 regulates T/B cell receptor signaling, T cell activation, T helper cell differentiation, cytokine production, actin polymerization, and cytoskeleton reorganization via GEF-dependent and -independent pathways. Recent studies suggest that targeting VAV1 has therapeutic potential in T and T/B cell-mediated autoimmune and chronic inflammatory diseases (1, 2).

Recombinant human VAV1 protein with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).

Tag(s): The N-terminal 6XHis and AviTag
Amino Acids: 178-574 of VAV1 (UniProt # P15498-1).

Expression system: E.coli

Catalog # VAV1-B-301

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 5 mM 2-mercaptoethanol, 0.04 % Tween 20, 20% Glycerol. 
Concentration: 1.0 mg/mL by A280 (please see specific lot number).

Molecular weight: ~49.7 kDa
Purity: >85% by Coomassie staining 
Storage is recommended at -80°C. 

International Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.

For Bulk Orders or Custom Packaging:  please contact info@amidbiosciences.com

This product is for laboratory research use only.

References

1.   Neurath, Markus F. et al. VAV1 as a putative therapeutic target in autoimmune and chronic inflammatory diseases. Trends in Immunology 2024, 45 (8), 580 – 596. 

2.   Guerreiro-Cacais, A.O. et al. VAV1 regulates experimental autoimmune arthritis and is associated with anti-CCP negative rheumatoid arthritis. Genes Immun. 2017; 18:109.