Neurofibromin (NF1), human, recombinant

Neurofibromin, the protein encoded by the NF1 tumor-suppressor gene, is a GTPase activating protein (GAP) of Ras (Ras-GAP). It downregulates the Ras signaling pathway by promoting the hydrolysis of the active form of Ras (GTP-bound Ras) to an inactive form of Ras (GDP-bound Ras) by increasing the intrinsic GTPase activity of Ras (1).

A GTPase Activating Domain from the human neurofibromatosis type 1 gene, NF1 (amino acids 1197-1530) with a polyhistidine tag at the N-terminus was expressed in E. coli.

Catalog # NF1-301

Expression host: E. coli

Tag(s): The N-terminal 6XHis

Amino Acids: 1197-1530 of human NF1 (UniProt # P21359)

Storage buffer: 20 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, 10% Glycerol. 
Concentration: 1.0 - 2.0 mg/mL by A280 (please see specific lot number).

Molecular weight: ~39.3 kDa
Purity: >90% by Coomassie staining 
Storage is recommended at -80°C for longer periods of time. 

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.

For Bulk Orders or Custom Packaging:  please contact info@amidbiosciences.com

This product is for laboratory research use only.

References

1. Bergoug M, Doudeau M, Godin F, Mosrin C, Vallée B, Bénédetti H. Neurofibromin Structure, Functions and Regulation. Cells. 2020 Oct 27;9(11):2365.