Amid Biosciences | Protein Engineering Company
KRAS Q61H Protein, Human, Recombinant, AviTag
Recombinant KRAS Q61H protein with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells. Presence of AviTag™ peptide can be useful for a site-specific biotinylation with BirA enzyme (https://amidbiosciences.com/collections/biotinylation-tools-streptavidins/products/bira-biotin-ligase-in-vitro-biotynilation).
Sequence of recombinant human KRAS Q61H protein (amino acids 1 – 185; Q61H mutant variant of isoform KRAS4B (UNIPROT: P01116-2))
Loading of the KRAS proteins with nucleotides or nucleotides' analogs can be performed if requested.
Catalog # KRAS61H-301
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Storage buffer: 20 mM HEPES, pH 7.5, 250 mM NaCl, 1 mM MgCl2, 5 mM 2-mercapthoethanol, 10% glycerol.
Concentration: 1.0 mg/mL by A280
Purity: >90% by Coomassie staining
Storage is recommended at -20°C for longer periods of time.
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This product is for laboratory research use only.
KRAS Protein Structure Image from National Cancer Institute (NCI): https://visualsonline.cancer.gov/details.cfm?imageid=11166