Amid Biosciences | Protein Engineering Company
HRV 3C Protease, Recombinant
Amid Biosciences HRV 3C protease is a recombinant cysteine protease derived from human Rhinovirus type 14. The enzyme is known as PreScission Protease. HRV 3C protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro. Besides the recognition sequence, the secondary and tertiary structures of the fusion protein influence on substrate recognition and cleavage.
The recombinant enzyme is a 47.9 kDa fusion protein with glutathione-S-transferase (GST) and polyhistidine (6xHis) tags, so it can be removed from the protease reaction solution through immobilized metal affinity chromatography (IMAC) or using immobilized glutathione (GSH) resin.
- Low temperature cleavage of fusion proteins.
- Active in a variety of commonly used buffers.
- On-column or off-column cleavage
- Removal of purification tags from fusion proteins.
Concentration: 0.8-1.2 mg/ml
One unit of HRV 3C Protease is defined as the amount of enzyme that cleaves ≥95% of the control fusion protein (0.1 mg in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5, and 1 mM DTT) in 16 hours at 4°C.
Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM DTT and 50% (V/V) glycerol.
Storage: at -20°C.
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This product is intended for laboratory research use only.