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Streptavidin Recombinant | High Purity | Diagnostic Applications | Protein-Protein Interaction

Amid Biosciences| Competent Cells and Protein Expression Vectors

Streptavidin Recombinant | High Purity

$ 500.00

Streptavidin is a homo-tetrameric protein secreted by Streptomyces avidinii. Streptavidin is used extensively in molecular biology for its extraordinarily high affinity for biotin.  The binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature with a dissociation constant (Kd) of the biotin-streptavidin complex on the order ~10-14 mol/L. The streptavidin/biotin complex is extremely stable over a wide range of temperature and pH. Because streptavidin lacks any carbohydrate modification and has a near-neutral pI, it has the advantage of much lower nonspecific binding than avidin.
Recombinant Streptavidin is produced in E. coli as an N- and C-terminal shortened variant (core streptavidin, amino acids 13-139) and purified by proprietary chromatographic techniques. A single, non-glycosylated polypeptide chain contains a total of 128 amino acids with a molecular mass of 13.4 kDa. The molecular weight per tetramer is approximately 53.6 kDa.

Streptavidin sequence:  
MAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS 

The Streptavidin solution (5 mg/ml) contains 0.5XPBS buffer, pH 7.4 and 50% glycerol. 

SKU: ST-302-5 (5 mg), ST-302-25 (25 mg)

Activity: Greater than 16 U/mg, 1 unit binds 1 µg of d-biotin.
Storage is recommended at -20°C for longer periods of time. Minimize freeze/thaw cycles.  

International Shipping:  Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates

This product is for laboratory research use only.

Application References:

1. Dundas et. al., Streptavidin-biotin technology: improvements and innovations in chemical and biological applications. Appl Microbiol Biotechnol. 2013 97(21): 9343.

 


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