Protein A Magnetic Nanoparticles

Amid Biosciences Protein A functionalized magnetic nanoparticles  – Protein A SilaMag Nanoparticles (Catalog # PAMP-101-1) are high-capacity affinity particles for antibody purification and immunoprecipitation methods. Protein A SilaMag Nanoparticles with average size of 400 nm are made according to a modified Stoeber process by hydrolysis of orthosilicates and consecutive coupling with recombinant Protein A. Recombinant Protein A contains five high-affinity IgG binding domains. 

Applications:

• Affinity purifications of IgG antibodies from various sources.
• Immunoprecipitation for protein isolation.
• Co-immunoprecipitation (Co-IP) or protein complex pull-down.

Features:

• High-affinity for for Fc region of IgG antibodies from human and mouse.
• High binding capacity (greater than > 25 µg of Human IgG per mg of particles).
• Binds to total IgGs from cow, guinea pig, hamster, horse, pig, and rabbit.
• Fast magnetic separation.

Catalog # PAMP-101-1 

Protein A SilaMag Nanoparticles supplied as 10 mg/ml in 1X PBS, pH 7.4, 0.05% NaN₃, 0.05% Tween 20.

Precautions and Disclaimer

Protein A SilaMag Nanoparticles are for laboratory use only. The storage buffer contains 0.05% sodium azide which is toxic. Please refer to the Safety Data Sheet (SDS).

Storage/Stability

The suspension should be stable approximately six months stored at 2-8 °C. DO NOT FREEZE. Freezing will damage bead structure.

International Shipping:  Product requires shipping on ice packs. Please contact info@amidbiosciences.com for shipment estimates. 

For custom orders please contact info@amidbiosciences.com