Phage Display TG1 Chemically Competent E. coli Cells

TG1 chemically competent cells can be used for phage display, antibody phage display library construction and screening, cloning, sub-cloning, and protein expression. lacI^q gene encoded on F’ episome allows tight regulation of expression vectors that contain an E. coli promoter under control of a lac operator sequence, like T5, tac, trc.

TG1 chemically competent cells are supplied as a pack of 10 convenient (100 µl/tube) aliquots sufficient for 20 reactions.

Electrocompetent TG1 E.coli cells are also available. 

Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells. 

Catalog # TG1-201

Genotype:

F’[traD36 lacI^q lacZ ∆M15 proA⁺B⁺] glnV (supE) thi-1 ∆(mcrB-hsdSM)5 (r_K^- m_K^- McrB^-) ∆(lac-proAB)

Features and Benefits

• Transformation efficiency > 10⁸transformants /µg pBR322
• Useful for preparation of phage display libraries
• Fast-growing
• Amber suppressor (supE): UAG>CAG(Gln)
• Appropriate for blue/white screening by α-complementation

Transformation Protocol

Storage:  at -80°C.

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Application References

1. Clackson et al. Making antibody fragments using phage display libraries. (1991) Nature 352: 624

2. Pini et al. Design and Use of a Phage Display Library. Human Antibodies with Subnanomolar Affinity against a Marker of Angiogenesis Eluted from a Two-Dimensional Gel. (1998) JBC 273: 21769