Amid Biosciences | Protein Engineering Company
BL21(DE3) pLPP – Lambda Protein Phosphatase Co-Expression System | Production of Unphosphorylated Proteins
Undesired phosphorylation of proteins can be observed during expression in various strains due to either phosphorylation by host kinases or autophosphorylation.
The BL21(DE3) pLPP is an E. coli strain that is developed for high-efficiency expression of homogeneously non-phosphorylated proteins including both serine/threonine kinases and tyrosine kinases. The BL21(DE3) pLPP competent cells contain an IPTG-inducible bacteriophage lambda protein phosphatase (lambda PP) expression plasmid. Lambda protein phosphatase is highly specific enzyme that removes phosphorylation from Ser, Thr, and Tyr residues in proteins (1-3). The lambda PP plasmid carries the resistance gene for streptomycin/spectinomycin and is compatible for co-expression with many E. coli and T7 promoter driven vectors (pET, pTrc, and etc).
Amid Biosciences BL21(DE3) pLPP chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.
Catalog# BLPP-201, Size: 10 X 0.1 ml/tube
Type: Chemically Competent Cells; Transformation efficiency: >1 x 104 cfu/µg pBR322
E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS pLPP (StrR)
[λDE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Storage: at -80°C
Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
References
1. Bhoir, S., Shaik, A., Thiruvenkatam, V. et al. High yield bacterial expression, purification and characterization of bioactive Human Tousled-like Kinase 1B involved in cancer. Sci Rep 8, 4796 (2018). https://doi.org/10.1038/s41598-018-22744-5
3. Shrestha, A. et al. Analysis of conditions affecting auto-phosphorylation of human kinases during expression in bacteria. Protein Exp. and Purification, 2012, 81 (1), 136-143. https://doi.org/10.1016/j.pep.2011.09.012
