Amid Biosciences| Competent Cells and Protein Expression Vectors
BL21(λDE3) ΔserC ΔycdX Chemically Competent Cells for In Vivo Incorporation of Phosphothreonine
The BL21(λDE3) ΔserC ΔycdX is an engineered strain for efficient co-translational incorporation of phosphothreonine (pThr) into recombinant proteins by an Escherichia coli. In this strain, deletion of serC gene which is responsible for the intracellular levels of phosphoserine along with deletion of a putative phosphatase, ycdX, enables homogeneous pThr incorporation at different sites in different proteins (Zhang et al.,2017).
BL21(λDE3)ΔserC ΔycdX chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots. The transformation efficiency is ≥ 1x106 cfu/μg pBR322 plasmid DNA.
E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC ΔycdX
[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]
Catalog # BLPCY-201
Storage: - 80°C.
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Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Important Note: Purchase of BL21(λDE3) ΔserC ΔycdX cells includes research use limited license for use only at site of purchase. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences LLC. Please review this license at our Legal Communique Section before purchasing this product.
Product Description and Manual
Zhang MS,et al. Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing. Nat Methods. 2017 Jul;14(7):729-736. doi: 10.1038/nmeth.4302. Epub 2017 May 29.