Amid Biosciences
Combo Pack of AB 5-alpha and BL21(DE3) Chemically Competent E.coli Cells
This Chemically Competent E.coli Cells Combo Pack allows fulfilling your cloning and protein expression needs. The Combo Pack contains 5 tubes of AB 5-alpha and 5 tubes of BL21(DE3) chemically competent E. coli cells in convenient packaging format (100 μl/tube aliquots).
AB 5-alpha cells are directly comparable to DH5a™ cells. These cells are perfect for use in all cloning applications, including preparation of high-quality plasmid DNA. They offer efficient transformation even with large plasmids.
The transformation efficiency is ≥ 1x108 cfu/μg pUC19 plasmid DNA.
Applications
- Transformation of plasmids into bacterial cells
- Cloning, sub-cloning, library construction
- Blue/white color screening
Catalog # CAB5BL21-201
Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-
BL21(DE3) chemically competent E.coli cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs.
Compatible Expression Vectors/Promoters: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T7
The transformation efficiency is ≥ 1x106 cfu/μg pUC19 plasmid DNA.
Applications
- Ideal for expressing proteins in E. coli.
- BL21(DE3) cells carry the lambda DE3 lysogen for expression from vectors utilizing the T7 promoter.
Genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS.
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Storage: -80°C. Thaw on ice only before use. Do not re-freeze.
Shipping: Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.
Usage:
This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
AB 5-alpha Transformation Protocol
BL21(DE3) Transformation Protocol
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells.
