BL21 (DE3) Electrocompetent E. coli Cells | High Level Recombinant Protein

BL21(DE3) electrocompetent E. coli cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs. BL21 (DE3) electrocompetent cells are supplied as a pack of 10 single-use 50 μl/tube aliquots.The transformation efficiency is ≥ 1x10⁸ cfu/µg pBR322 plasmid DNA.

Catalog # EBL21-201

 E. coli genotype:

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Quality Control Testing: Transformations are performed using 50 μl aliquots of cells and 50 pg of pUC19 control plasmid. 

Storage: Transfer the cells directly from the dry ice shipping container to the –80°C freezer.

BL21 (DE3) Chemically Competent E.coli cells are also available.

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Electrocompetent BL21 (DE3) electroporation protocol.

Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells.