RalA Protein, Human, Recombinant, Biotinylated

Ras-related protein Ral-A (RALA) is a member of the Ras GTPase family and shares a high structural similarity with RAS proteins (1). In human cells, the two protein isoforms, RALA and RALB, have been shown to play roles in the proliferation, survival, and metastasis of a variety of human cancers. RALA and RALB share 81% sequence homology with one another and more than 50% sequence homology with RAS. Given their importance in cancer biology, RAL proteins have emerged as desirable clinical targets (2).

Recombinant RalA protein with the N-terminal AviTag™ peptide and His-tag is produced in E. coli cells and site-specifically biotinylated at AviTag with BirA enzyme (https://amidbiosciences.com/collections/proteins/products/bira-biotin-ligase).

Tag(s): The N-terminal 8XHis and AviTag
Amino Acids: 1-206 of RalA (UniProt # P11233)

Catalog # RALA-B-301

Storage buffer: 20 mM Tris-HCl, pH 7.5, 100 mM NaCl, 2 mM DTT, 10% Glycerol. 
Molecular weight: ~27 kDa
Purity: >90% by Coomassie staining 
Storage is recommended at -80°C. 

International Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates.

For Bulk Orders or Custom Packaging:  please contact info@amidbiosciences.com

This product is for laboratory research use only.

References

1. Yan, C., Theodorescu, D. RAL GTPases: Biology and Potential as Therapeutic Targets in Cancer. Pharmacol Rev. 2018, 70: 1–11; https://doi.org/10.1124/pr.117.014415
2. Richardson, D. et al. The RAL Enigma: Distinct Roles of RALA and RALB in Cancer. Cells 2022, 11(10), 1645; https://doi.org/10.3390/cells11101645