SARS-CoV-2 Spike S1 Protein, Recombinant, AviTag

The coronavirus Spike protein (S protein) is a large oligomeric transmembrane protein that mediates coronavirus entry into host cells. It contains S1 and S2 subunits. The coronavirus first binds to a receptor on the host cell surface through Spike S1 subunit, and then fuses viral and host membranes through Spike S2 subunit. S1 subunit contains N-terminal domain (NTD), receptor binding domain (RBD), and receptor binding motif (RBM).

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with the 8XHis tag and AviTag at the N-terminus.

Catalog number: COVS1-301-1 (0.1 mg); COVS1-301-2 (1.0 mg)

Expression host: Escherichia coli (E. coli).

Species: SARS-CoV-2

Expressed Region of S1: Val16 – Arg685

Predicted molecular weight: 78.1 kDa

Tags: His, AviTag

Purity: > 90% as analyzed by SDS-PAGE

Storage buffer: 10 mM Tris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT and 50% Glycerol. 

Storage: The product can be stored at -20°C or below. Avoid repeated freezing and thawing cycles. The shelf life of the product is unspecified.

This product is intended for laboratory research use only. 

Image of 3D print of a spike protein on the surface of SARS-CoV-2 (Credit: the NIH 3D Print Exchange at 3dprint.nih.gov; NIH)