T7 RNA Polymerase

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for its cognate promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript from a DNA sequence cloned downstream of the T7 promoter.  

Applications

• Synthesis of mRNA for in vitro translation.
• Synthesis of guide RNA.
• Generation of antisense RNA.
• Generation of probes for nucleic acid hybridizations.

Storage buffer: 40 mM Tris-HCl (pH 7.9 at 25°C), 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% (v/v) Triton® X-100 and 50% glycerol.

The enzyme is supplied with 10X T7 RNA Polymerase Buffer (400 mM Tris-HCl, pH 7.9, 60 mM MgCl₂, 100 mM Dithiothreitol, 20 mM Spermidine).

Catalog number: T7RP-301

Concentration: 50,000 U/ml

Unit Definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of CTP into acid-insoluble material in 1 hour at 37°C and pH 8.0 using linear DNA as the substrate.

Source: Purified from a strain of E. coli that expresses the recombinant T7 RNA Polymerase gene.

Storage: –20 °C

Usage: For laboratory research use only.