{"product_id":"recombinant-bira-biotin-ligase-in-vitro-biotinylation","title":"BirA Biotin Protein Ligase, Recombinant","description":"\u003ch2\u003eRecombinant BirA Biotin Ligase (EC 6.3.4.15)\u003c\/h2\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003eBirA (also known as biotin protein ligase or BPL) is widely used for antibody biotinylation, proximity-dependent biotinylation (BioID), and streptavidin\/avidin-based pull-down assays. Because biotinylation is confined to the AviTag sequence (GLNDIFEAQKIEWHE), it avoids the non-specific labeling seen with chemical NHS-ester reagents, preserving protein activity and enabling oriented capture on streptavidin surfaces. \u003cmeta charset=\"utf-8\"\u003eUnlike chemical NHS-ester methods that produce heterogeneous mixtures, BirA achieves site-specific biotinylation exclusively at the AviTag lysine residue.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eKey Features \u0026amp; Specifications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource \u0026amp; Expression:\u003c\/strong\u003e Expressed in \u003cem\u003eE. coli\u003c\/em\u003e cell expression system (amino acids 3–321).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMolecular Weight:\u003c\/strong\u003e 36.2 kDa.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification Tag:\u003c\/strong\u003e Features an N-terminal His-tag, allowing for easy removal post-reaction using a Ni-NTA column (if the substrate lacks a His-tag).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;90% as determined by SDS-PAGE (Coomassie staining).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Activity:\u003c\/strong\u003e 5,000 Units\/µg. (1 Unit biotinylates 1 pmol of AviTag peptide substrate in 30 minutes at 30°C).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConcentration:\u003c\/strong\u003e 1.0 mg\/mL (by A280).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications:\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eSite-specific \u003cem\u003ein vitro\u003c\/em\u003e biotinylation of target proteins and antibodies.\u003c\/li\u003e\n\u003cli\u003eProximity-dependent biotinylation and cell labeling applications.\u003c\/li\u003e\n\u003cli\u003ePreparation of high-affinity complexes for streptavidin\/avidin pull-downs, immobilization, and functionalization assays.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Amid Biosciences","offers":[{"title":"100 ug","offer_id":12268311478353,"sku":"BBL-301-100","price":240.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BirA_Biotin_Protein_Ligase_Recombinant.png?v=1779579481","url":"https:\/\/amidbiosciences.com\/products\/recombinant-bira-biotin-ligase-in-vitro-biotinylation","provider":"Amid Biosciences","version":"1.0","type":"link"}