{"product_id":"phi29-dna-polymerase","title":"phi29 DNA Polymerase","description":"\u003ch3\u003eHigh-Processivity Replicative Polymerase\u003c\/h3\u003e\n\u003cp\u003ephi29 DNA Polymerase is a highly processive replicative polymerase derived from the \u003cem\u003eBacillus subtilis\u003c\/em\u003e phage phi29 (Φ29). It features an extraordinary strand displacement capacity, synthesizing up to 70 kb per single binding event with exceptional speed and efficiency.\u003c\/p\u003e\n\u003ch3\u003eExceptional Synthesis Fidelity\u003c\/h3\u003e\n\u003cp\u003eEquipped with robust 3' → 5' proofreading exonuclease activity, this polymerase ensures an exceptionally high level of fidelity during long DNA synthesis workflows.\u003c\/p\u003e\n\u003ch3\u003eProduct Specifications \u0026amp; Storage\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurity:\u003c\/strong\u003e \u0026gt;95% based on SDS-PAGE verification.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSource:\u003c\/strong\u003e Recombinant \u003cem\u003eE. coli\u003c\/em\u003e strain expressing the bacteriophage phi29 gene.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage Buffer:\u003c\/strong\u003e 10 mM Tris-HCl (pH 7.4 @ 25°C), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween-20, 0.5% NP-40, and 50% glycerol.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReaction Buffer (10X):\u003c\/strong\u003e 500 mM Tris-HCl (pH 7.5 @ 25°C), 100 mM (NH\u003csub\u003e4\u003c\/sub\u003e)\u003csub\u003e2\u003c\/sub\u003eSO\u003csub\u003e4\u003c\/sub\u003e, 40 mM DTT, and 100 mM MgCl\u003csub\u003e2\u003c\/sub\u003e.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003eUnit Definition:\u003c\/strong\u003e 1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dNTPs into acid-insoluble material in 10 minutes at 30°C. Catalog# PHDP-301. \u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e","brand":"Amid Biosciences | Protein Engineering Company","offers":[{"title":"1000 Units","offer_id":40287236620369,"sku":"PHDP-301-1","price":140.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/phi69_DNA_Polymerase.png?v=1736368840","url":"https:\/\/amidbiosciences.com\/products\/phi29-dna-polymerase","provider":"Amid Biosciences","version":"1.0","type":"link"}