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Electrocompetent BL21-DE3 E. coli cells high level protein expression | Amid Biosciences

Amid Biosciences

BL21 (DE3) Electrocompetent E. coli Cells | Amid Biosciences

$ 100.00

BL21(DE3) electrocompetent E. coli cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs. BL21 (DE3) electrocompetent cells are supplied as a pack of 10 single-use 50 μl/tube aliquots.The transformation efficiency is ≥ 1x108 cfu/µg pBR322 plasmid DNA.

Catalog # EBL21-201

 E. coli genotype:

fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS. [λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Quality Control Testing: Transformations are performed using 50 μl aliquots of cells and 50 pg of pUC19 control plasmid. 

Storage: Transfer the cells directly from the dry ice shipping container to the –80°C freezer.

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Electrocompetent BL21 (DE3) electroporation protocol.

BL21 (DE3) Chemically Competent E.coli cells are also available.

Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells. 


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