Combo Pack of AB 5-alpha and BL21(DE3) Competent Cells | Amid Biosciences
This economical Competent Cells Combo Pack allows fulfilling your cloning and protein expression needs. The Combo Pack contains 5 tubes of AB 5-alpha and 5 tubes of BL21(DE3) chemically competent E. coli cells in convenient packaging format (100 μl/tube aliquots).
AB 5-alpha cells are directly comparable to DH5a™ cells. These cells are perfect for use in all cloning applications, including preparation of high-quality plasmid DNA. They offer efficient transformation even with large plasmids.
The transformation efficiency is ≥ 1x108 cfu/μg pUC19 plasmid DNA.
- Transformation of plasmids into bacterial cells
- Cloning, sub-cloning, library construction
- Blue/white color screening
Catalog # CAB5BL21-201
Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-
BL21(DE3) chemically competent cells are suitable for transformation, high-level protein expression and production of recombinant proteins in bacterial systems. The strain lacks the lon and ompT proteases thus promoting stability of recombinant proteins. BL21(DE3) strain carries a copy of the T7 RNA polymerase under control of the IPTG inducible lacUV5 promoter, and as a result, is ideal for controlled expression of both E. coli and T7 promoter driven constructs.
Compatible Expression Vectors/Promoters: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T7
The transformation efficiency is ≥ 1x106 cfu/μg pUC19 plasmid DNA.
- Ideal for expressing proteins in E. coli.
- BL21(DE3) cells carry the lambda DE3 lysogen for expression from vectors utilizing the T7 promoter.
Genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS.
λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5
Storage: -80°C. Thaw on ice only before use. Do not re-freeze.
Shipping: Product requires shipping on dry ice. Please contact firstname.lastname@example.org for shipment estimates.
This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Bulk quantities and custom packaging are available at very competitive prices for all Competent Cells.