{"product_id":"bl21-de3pbira-e-coli-cells-for-in-vivo-biotinylation","title":"BL21(λDE3)pBirA Competent Cells for in vivo Biotinylation","description":"\u003cblockquote\u003e\n\u003cp\u003eThe \u003cb\u003eBL21(λDE3)pBirA strain\u003c\/b\u003e is highly recommended for the expression and \u003ci\u003ein vivo\u003c\/i\u003e biotinylation of target proteins fused with an \u003cb\u003eAviTag™ peptide\u003c\/b\u003e or other specific peptide sequences recognized by \u003ci\u003eE. coli\u003c\/i\u003e BirA biotin ligase.\u003c\/p\u003e\n\u003cp\u003eThis strain features an IPTG-inducible BirA expression plasmid carrying a streptomycin\/spectinomycin resistance gene. It enables high-efficiency protein expression of any gene under the control of a \u003cb\u003eT7 or E. coli promoter\u003c\/b\u003e equipped with a ribosome binding site. The BirA plasmid is fully compatible for co-expression with popular \u003ci\u003eE. coli\u003c\/i\u003e and T7 promoter-driven vectors (including pET and pTrc).\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eApplications \u0026amp; Usage:\u003c\/b\u003e After transforming the BL21(λDE3)pBirA chemically competent \u003ci\u003eE. coli\u003c\/i\u003e cells with your target vector (carrying an AviTag™ sequence and an antibiotic resistance gene other than streptomycin\/spectinomycin), grow the cells in the presence of both selection antibiotics. Induce BirA expression using \u003cb\u003eIPTG\u003c\/b\u003e, and add exogenous biotin to the media at the time of induction at a final concentration of 50 to 200 µM.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cb\u003eCatalog #:\u003c\/b\u003e BLB-201\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cmeta charset=\"utf-8\"\u003e\n\u003cp\u003e\u003cstrong\u003eTransformation efficiency:\u003c\/strong\u003e ≥ 1x10^6 cfu\/μg pBR322 plasmid DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cb\u003eIntended Use:\u003c\/b\u003e\u003cstrong\u003e For laboratory research use only (RUO).\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/blockquote\u003e","brand":"Amid Biosciences| Competent Cells and Protein Expression Vectors","offers":[{"title":"10x0.1 ml (20 reactions)","offer_id":21302001336401,"sku":"BLB-201","price":340.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BL21BirA.jpg?v=1779571526","url":"https:\/\/amidbiosciences.com\/products\/bl21-de3pbira-e-coli-cells-for-in-vivo-biotinylation","provider":"Amid Biosciences","version":"1.0","type":"link"}