{"product_id":"bl21-de3-serc-ycdx-competent-cells","title":"BL21(λDE3)ΔserC ΔycdX Chemically Competent Cells | Amid Biosciences","description":"\u003ch2 data-path-to-node=\"3\"\u003e\u003cb data-path-to-node=\"3\" data-index-in-node=\"0\"\u003eBL21(DE3) ΔserC ΔycdX Chemically Competent Cells\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp data-path-to-node=\"4\"\u003eThe \u003cb data-path-to-node=\"4\" data-index-in-node=\"4\"\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003ca rel=\"noopener\" href=\"https:\/\/www.google.com\/search?q=https:\/\/merchants.google.com\/mc\/items%3Fa%3D5776980113%26tableState%3DEhkKFWNhbGN1bGF0ZWRfdmlzaWJpbGl0eRABGDI%253D\" class=\"ng-star-inserted\" data-hveid=\"0\" data-ved=\"0CAAQ_4QMahgKEwjZ7smo_6KUAxUAAAAAHQAAAAAQpQM\" target=\"_blank\"\u003eBL21(DE3) ΔserC ΔycdX\u003c\/a\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c!----\u003e\u003c\/b\u003e strain is a highly engineered \u003ci data-path-to-node=\"4\" data-index-in-node=\"56\"\u003eE. coli\u003c\/i\u003e host optimized for advanced protein engineering and the site-specific incorporation of non-natural amino acids. This strain is specifically designed to enhance the yield and purity of recombinant proteins containing synthetic modifications.\u003c\/p\u003e\n\u003ch3 data-path-to-node=\"5\"\u003e\u003cb data-path-to-node=\"5\" data-index-in-node=\"0\"\u003eTechnical Specifications \u0026amp; Mechanism\u003c\/b\u003e\u003c\/h3\u003e\n\u003cul data-path-to-node=\"6\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,0,0\"\u003e\u003cb data-path-to-node=\"6,0,0\" data-index-in-node=\"0\"\u003eGenotype:\u003c\/b\u003e BL21(DE3) background with targeted deletions of both \u003cb data-path-to-node=\"6,0,0\" data-index-in-node=\"63\"\u003eserC\u003c\/b\u003e and \u003cb data-path-to-node=\"6,0,0\" data-index-in-node=\"72\"\u003eycdX\u003c\/b\u003e genes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,1,0\"\u003e\u003cb data-path-to-node=\"6,1,0\" data-index-in-node=\"0\"\u003eEngineered Pathway:\u003c\/b\u003e\u003c\/p\u003e\n\u003cul data-path-to-node=\"6,1,1\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,1,1,0,0\"\u003e\u003cb data-path-to-node=\"6,1,1,0,0\" data-index-in-node=\"0\"\u003eserC Deletion:\u003c\/b\u003e Disrupts the endogenous synthesis of phosphoserine, reducing competitive interference during the translation of recombinant proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,1,1,1,0\"\u003e\u003cb data-path-to-node=\"6,1,1,1,0\" data-index-in-node=\"0\"\u003eycdX Deletion:\u003c\/b\u003e Removes specific phosphatase activity to further stabilize the intracellular levels of synthetic analogs.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"6,2,0\"\u003e\u003cb data-path-to-node=\"6,2,0\" data-index-in-node=\"0\"\u003ePrimary Application:\u003c\/b\u003e Optimized for the incorporation of \u003cb data-path-to-node=\"6,2,0\" data-index-in-node=\"56\"\u003ephosphothreonine\u003c\/b\u003e and \u003cb data-path-to-node=\"6,2,0\" data-index-in-node=\"77\"\u003ephosphoserine\u003c\/b\u003e analogs into target proteins for structural and functional analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\n\u003cmeta charset=\"utf-8\"\u003eValidated Applications \u0026amp; Recent Citations:\u003c\/h3\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cspan\u003eAllen MC, Karplus PA, Mehl RA, Cooley RB. Genetic Encoding of Phosphorylated Amino Acids into Proteins. \u003c\/span\u003e\u003ci\u003eChem Rev\u003c\/i\u003e\u003cspan\u003e. 2024;124(10):6592-6642. doi:\u003ca href=\"https:\/\/pubs.acs.org\/doi\/10.1021\/acs.chemrev.4c00110\" title=\"Genetic Encoding of Phosphorylated Amino Acids into Proteins\" rel=\"noopener\" target=\"_blank\"\u003e10.1021\/acs.chemrev.4c00110\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-path-to-node=\"8,0,0\"\u003e\u003cb data-path-to-node=\"8,0,0\" data-index-in-node=\"0\"\u003eResearch Use Only (RUO):\u003c\/b\u003e This product is intended strictly for laboratory research and development purposes. It is not approved for use in clinical diagnostics, therapeutic procedures, or any human or animal applications.\u003c\/p\u003e","brand":"Amid Biosciences| Competent Cells and Protein Expression Vectors","offers":[{"title":"10 × 0.1 mL","offer_id":59718504317009,"sku":"BLPCY-201","price":2600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/BL21invivoP.jpg?v=1745007545","url":"https:\/\/amidbiosciences.com\/products\/bl21-de3-serc-ycdx-competent-cells","provider":"Amid Biosciences","version":"1.0","type":"link"}