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Protein Phosphorylation | Protein Expression | Phosphothreonine | Amid Biosciences

Amid Biosciences| Competent Cells and Protein Expression Vectors

BL21(λDE3) ΔserC ΔycdX Chemically Competent Cells for In Vivo Incorporation of Phosphothreonine I Amid Biosciences

$ 1,500.00

The BL21(λDE3) ΔserC ΔycdX is an engineered strain for efficient co-translational incorporation of phosphothreonine (pThr) into recombinant proteins by an Escherichia coli. In this strain, deletion of serC gene which is responsible for the intracellular levels of phosphoserine along with deletion of a putative phosphatase, ycdX, enables homogeneous pThr incorporation at different sites  in different proteins (Zhang et al.,2017).

BL21(λDE3)ΔserC ΔycdX chemically competent cells are supplied as a pack of 10 convenient 100 μl/tube aliquots.  The transformation efficiency is ≥ 1x106 cfu/μg pBR322 plasmid DNA.

E. coli genotype: fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS ΔserC ΔycdX

[λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5]

Catalog # BLPCY-201

Storage: - 80°C. 

Shipping:  Product requires shipping on dry ice. Please contact info@amidbiosciences.com for shipment estimates. 

Usage: This product is intended for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

Important Note: Purchase of BL21(λDE3) ΔserC ΔycdX cells includes a license for use only at site of purchase and may not be distributed to other sites.

License: The BL21(λDE3) ΔserC ΔycdX Strain when purchased conveys a limited use license to the end user. This license prohibits the purchaser from selling, assigning or transferring this product to any third party without the express written consent of Amid Biosciences, LLC. Please review this license before purchasing this product.

References

Zhang MS,et al. Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing. Nat Methods. 2017 Jul;14(7):729-736. doi: 10.1038/nmeth.4302. Epub 2017 May 29.


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