{"title":"Transposases","description":"\u003cp\u003eBacterial \"cut-and-paste\" transposases, most notably hyperactive variants of \u003cstrong\u003eTn5 Transposase\u003c\/strong\u003e derived from \u003cem\u003eE. coli\u003c\/em\u003e, serve as the modern foundation for high-throughput \u003cstrong\u003eNGS tagmentation\u003c\/strong\u003e workflows. This highly efficient, single-tube method simultaneously fragments double-stranded DNA and tags it with sequencing adapters in a single rapid enzymatic step. By combining these reactions, tagmentation drastically reduces library preparation hands-on time and costs for whole-genome sequencing (WGS), ATAC-seq, and RNA-seq protocols.\u003c\/p\u003e\n\u003ch3\u003eKey Features \u0026amp; Advantages\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRobust Performance:\u003c\/strong\u003e Delivers exceptional enzymatic activity and highly uniform sequence coverage across diverse genomic templates.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStreamlined Workflows:\u003c\/strong\u003e Eliminates the need for separate physical or enzymatic DNA shearing and subsequent adapter ligation steps.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlexible Formats:\u003c\/strong\u003e Available as an \u003ca href=\"\/products\/amtag-tn5-transposase-unloaded\"\u003eunloaded Tn5 transposase\u003c\/a\u003e for custom adapter design and barcoding, as well as pre-loaded transposome complexes for standardized protocols.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlatform Compatibility:\u003c\/strong\u003e Optimized for seamless integration with major short-read and next-generation sequencing platforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eNext-Generation Sequencing (NGS)\u003c\/strong\u003e library preparation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eATAC-seq\u003c\/strong\u003e (Assay for Transposase-Accessible Chromatin using sequencing)\u003c\/li\u003e\n\u003cli\u003eSingle-cell genomic and transcriptomic sequencing assays\u003c\/li\u003e\n\u003cli\u003eCustom cell-free DNA (cfDNA) and low-input DNA fragmentation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eOur high-purity, recombinant transposases undergo rigorous quality control testing to ensure reproducible fragmentation sizing distributions and high-yield final library construction.\u003c\/p\u003e","products":[{"product_id":"amtag-tn5-transposase-unloaded","title":"AMTAG Tn5 Transposase, Unloaded","description":"\u003cp data-path-to-node=\"3\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"3\"\u003eAMTAG Tn5 Transposase\u003c\/b\u003e is a hyperactive form of Tn5 transposase engineered for rapid, robust in vitro transposition. It enables researchers to randomly insert Tn5 transposons into any target DNA, streamlining workflows by combining fragmentation and adapter addition into a single, seamless step.\u003c\/p\u003e\n\u003cp data-path-to-node=\"4\"\u003eFor efficient transposition, the enzyme requires that each transposon contain a specific 19-bp transposase recognition sequence (Mosaic End or ME sequence: \u003ccode data-index-in-node=\"156\" data-path-to-node=\"4\"\u003e5’–CTGTCTCTTATACACATCT–3’\u003c\/code\u003e) at both ends. Upon binding, the enzyme forms a stable Transposome complex, catalyzing a random \"cut-and-paste\" reaction that yields a 9-bp sequence duplication immediately flanking the insertion site.\u003c\/p\u003e\n\u003cp data-path-to-node=\"5\"\u003eThis unloaded formulation provides ultimate flexibility, allowing you to load any custom adapter sequence or transposon DNA of any length for tailored next-generation sequencing (NGS) applications.\u003c\/p\u003e\n\u003ch3 data-path-to-node=\"6\"\u003eKey Applications\u003c\/h3\u003e\n\u003cul data-path-to-node=\"7\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"7,0,0\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"7,0,0\"\u003eCustom Transposome Assembly:\u003c\/b\u003e Flexible loading with user-defined oligonucleotide adapters.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"7,1,0\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"7,1,0\"\u003eNGS Library Preparation:\u003c\/b\u003e Efficient tagmentation for second-generation genomic sequencing, significantly reducing hands-on time and DNA input requirements compared to traditional methods.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-path-to-node=\"8\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cul data-path-to-node=\"9\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"9,0,0\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"9,0,0\"\u003eEnzyme Concentration:\u003c\/b\u003e Supplied at 1 U\/µl in a formulation containing 50% (v\/v) glycerol, corresponding to a high-purity protein concentration of \u003cb data-index-in-node=\"145\" data-path-to-node=\"9,0,0\"\u003e~17.5 µM\u003c\/b\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"9,1,0\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"9,1,0\"\u003eUnit Definition:\u003c\/b\u003e One unit (U) is defined as the amount of enzyme required to tagment 100 ng of human genomic DNA at 55 °C in 10 minutes into fragments ranging from 200 to 500 bp, as verified by agarose gel electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"9,2,0\"\u003e\u003cb data-index-in-node=\"0\" data-path-to-node=\"9,2,0\"\u003eStorage Conditions:\u003c\/b\u003e Store at -20 °C for long-term stability.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-path-to-node=\"10\"\u003eProduct Components\u003c\/h3\u003e\n\u003cul data-path-to-node=\"11\"\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"11,0,0\"\u003eAMTAG Tn5 Transposase, Unloaded\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"11,1,0\"\u003e10X Tn5 Reaction Buffer (100 mM Tris-HCl, 100 mM \u003cspan data-index-in-node=\"49\" data-math=\"MgCl_2\" class=\"math-inline\"\u003eMgCl_2\u003c\/span\u003e pH 7.5)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp data-path-to-node=\"11,2,0\"\u003e10X Stop Solution (0.5% SDS)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cspan\u003eCatalog Number: AMTN-301-20\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eCatalog Number: AMTN-301-100\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage: \u003c\/strong\u003e\u003cspan\u003eStore at -20 °C.\u003c\/span\u003e\u003c\/p\u003e","brand":"Amid Biosciences","offers":[{"title":"20 Units","offer_id":59560191361105,"sku":"AMTN-301-20","price":320.0,"currency_code":"USD","in_stock":true},{"title":"100 Units","offer_id":59560191393873,"sku":"AMTN-301-100","price":1280.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/files\/amtag-tn5-transposase-unloaded.png?v=1780340659"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0981\/2236\/collections\/AMTAG_Tn5_Transposase_Unloaded.png?v=1779686350","url":"https:\/\/amidbiosciences.com\/collections\/transposases.oembed","provider":"Amid Biosciences","version":"1.0","type":"link"}